| 產(chǎn)品編號 | bs-1253R |
| 英文名稱 | Rabbit Anti-AQP3 antibody |
| 中文名稱 | 水通道蛋白-3抗體 |
| 別 名 | AQP 3; AQP3; Aquaporin 3 (GIL blood group); Aquaporin 3 (Gill blood group); Aquaporin3; Aquaporin-3; GIL; Gill blood group; AQP3_MOUSE. |
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Specific References (8) | bs-1253R has been referenced in 8 publications.
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| 研究領(lǐng)域 | 腫瘤 信號轉(zhuǎn)導 通道蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 32kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse AQP3: 201-292/292 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. [provided by RefSeq, Aug 2011] Function: Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Renal medulla and colon. Predominantly in the inner medulla. Expressed in basal layer of epidermal keratinocytes. Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family. SWISS: Q8R2N1 Gene ID: 11828 Database links: Entrez Gene: 360 Human Entrez Gene: 11828 Mouse Omim: 600170 Human SwissProt: Q92482 Human SwissProt: Q8R2N1 Mouse Unigene: 234642 Human Unigene: 34043 Mouse Unigene: 11109 Rat 通道蛋白（Channel Protein） AQP 3主要分布于腎臟集合管上皮細胞基側(cè)質(zhì)膜,除了對水有通透性外、還可轉(zhuǎn)運尿素和甘油等小分子物質(zhì),而尿素在尿液濃縮機制中有著重要的作用. 有學者認為：AQP-3的陽性率與某些腫瘤分化程度有關(guān)（胃癌、結(jié)腸癌、乳腺癌、前列腺癌、子宮癌、卵巢癌、甲狀腺癌等） |
| 產(chǎn)品圖片 |
Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-AQP3 antibody (bs-1253R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control（black line）:A431.
Primary Antibody (green line): Rabbit Anti-AQP3 antibody (bs-1253R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
