| 產(chǎn)品編號 | bs-0004R |
| 英文名稱 | Rabbit Anti-ACTH (7-23) antibody |
| 中文名稱 | 促腎上腺皮質(zhì)激素ACTH (7-23)抗體 |
| 別 名 | Adrenocorticotropic hormone; Adrenocorticotropin; Adrenocorticotropin Hormone; Alpha Melanocyte Stimulating Hormone; Beta Endorphin; Beta Lipotropin; Beta Melanocyte Stimulating Hormone; CLIP; Corticotropin; Corticotropin Like Intermediary Peptide; Corticotropin lipotropin precursor; Lipotropin Beta; Lipotropin Gamma; LPH; Melanotropin Alpha; Met Enkephalin; MSH; NPP; POC; POMC; Pro opiomelanocortin; Proopiomelanocortin. |
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Specific References (3) | bs-0004R has been referenced in 3 publications.
[IF=7.081] Qingxing Liu. et al. Head-to-head comparison of 68Ga-DOTATATE PET/CT and 18F-FDG PET/CT in localizing tumors with ectopic adrenocorticotropic hormone secretion: a prospective study. 2021 Jun 19 IHC ; Human.
[IF=4.94] Zhou, Ding'an, et al. "A novel P53/POMC/Gαs/SASH1 autoregulatory feedback loop activates mutated SASH1 to cause pathologic hyperpigmentation." Journal of Cellular and Molecular Medicine (2016). IHC-P ; Human.
[IF=2.96] Jussila, Anna, et al. "Narrow-band ultraviolet B radiation induces the expression of β-endorphin in human skin in vivo." Journal of Photochemistry and Photobiology B: Biology (2016). IHC-P ; Human.
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| 研究領(lǐng)域 | 腫瘤 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 生長因子和激素 內(nèi)分泌病 腫瘤細(xì)胞生物標(biāo)志物 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Rabbit,Pig,Cow,Dog,GuineaPig) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 4.3kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ACTH: 7-23/39 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]. Function: ACTH stimulates the adrenal glands to release cortisol. MSH (melanocyte-stimulating hormone) increases thepigmentation of skin by increasing melanin production in melanocytes. Beta-endorphin and Met-enkephalin are endogenous opiates. Subunit: Belongs to the POMC family. Subcellular Location: Secreted. Tissue Specificity: ACTH and MSH are produced by the pituitary gland. DISEASE: Defects in POMC may be associated with susceptibility to obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD) [MIM:609734]. Affected individuals present early-onset obesity, adrenal insufficiency and red hair. Similarity: Belongs to the POMC family. SWISS: P01189 Gene ID: 5443 Database links: Entrez Gene: 5443 Human Entrez Gene: 18976 Mouse Omim: 176830 Human SwissProt: P01189 Human SwissProt: P01193 Mouse 促腎上腺皮質(zhì)激素(Adrenorticotrophin hormons, ACTH)是垂體前葉細(xì)胞分泌的一種多肽激素，是腎上腺皮質(zhì)活性的主要調(diào)節(jié)者。 此抗體可與人的ACTH反應(yīng),與多種其它哺乳動物的ACTH有交叉反應(yīng)，可用于垂體腺瘤的功能性分類，有助于區(qū)分原發(fā)性和轉(zhuǎn)移型垂體腫瘤，嗜絡(luò)細(xì)胞瘤等部分神經(jīng)內(nèi)分泌腫瘤也可出現(xiàn)陽性反應(yīng)。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Pituitary (Mouse) Tissue Lysate
Lane 2: Pituitary (Rat) Tissue Lysate
Primary:
Anti-ACTH (7-23) (bs-0004R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 4.3 kD
Observed band size: 31/22/13 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ACTH(7-23) Polyclonal Antibody, Unconjugated(bs-0004R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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