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Rabbit Anti-Perilipin-2  antibody (bs-1164R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號(hào) bs-1164R
英文名稱 Rabbit Anti-Perilipin-2  antibody
中文名稱 脂肪分化相關(guān)蛋白抗體
別    名 ADFP; Adipophilin; Adipose differentiation related protein; Adipose differentiation-related protein; PLIN2; PLIN2_HUMAN; adipophilin; Adipose differentiation related protein; ADRP; MGC10598  
Specific References  (4)     |     bs-1164R has been referenced in 4 publications.
[IF=8.47] Amrutkar, Manoj, et al. "Genetic Disruption of Protein Kinase STK25 Ameliorates Metabolic Defects in a Diet-Induced Type 2 Diabetes Model."Diabetes (2015).  Mouse.  
[IF=7.097] Yilan Shen et al. VEGF-B antibody and interleukin-22 fusion protein ameliorates diabetic nephropathy through inhibiting lipid accumulation and inflammatory responses.  IF ;  mouse.  
[IF=2.2] Liu et al. Oxidized low-density lipoprotein activates adipophilin through ERK1/2 signal pathway in RAW264.7 cells. (2010) Acta.Biochim.Biophys.Sin.(Shanghai. 42:635-45  WB ;  Mouse.  
[IF=1.851] Liu Q et al. Inhibitory effect of 17β?estradiol on triglyceride synthesis in skeletal muscle cells is dependent on ESR1 and not ESR2. Molecular Medicine Reports.2019,19: 5087-5096 .  Rat&Mouse.  
研究領(lǐng)域 腫瘤  心血管  細(xì)胞生物  免疫學(xué)  信號(hào)轉(zhuǎn)導(dǎo)  新陳代謝  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse (predicted: Rat)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 48kDa
細(xì)胞定位 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ADFP: 31-130/422 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]

Function:
May be involved in development and maintenance of adipose tissue.

Subcellular Location:
Membrane; Peripheral membrane protein.

Tissue Specificity:
Milk lipid globules.

Post-translational modifications:
Acylated; primarily with C14, C16 and C18 fatty acids.

Similarity:
Belongs to the perilipin family.

SWISS:
Q99541

Gene ID:
123

Database links:

Entrez Gene: 123 Human

Entrez Gene: 11520 Mouse

Entrez Gene: 298199 Rat

Omim: 103195 Human

SwissProt: Q99541 Human

SwissProt: P43883 Mouse

Unigene: 3416 Human

Unigene: 381 Mouse



Adipophilin(ADFP)是一種脂滴周圍主要的相關(guān)蛋白,它大量分布在脂質(zhì)蓄積正?;虿徽5募?xì)胞中,是脂質(zhì)蓄積的一個(gè)特異性標(biāo)記物。它在脂肪細(xì)胞分化早期就有很高的表達(dá),但當(dāng)脂肪細(xì)胞成熟后,它的表達(dá)就明顯減少,故稱;脂肪分化相關(guān)蛋白。
ADFP在很多組織器官中都發(fā)揮重要作用,它不僅參與脂肪細(xì)胞的脂質(zhì)代謝、脂滴的形成及肝內(nèi)三酰甘油(TO)的合成與代謝,還能促進(jìn)巨噬細(xì)胞、平滑肌細(xì)胞的泡沫化,長鏈脂肪酸的攝取,乳汁的分泌等。
ADFP的表達(dá)異常與動(dòng)脈粥樣硬化、胰島素抵抗和腫瘤等病理過程密切相關(guān)。
產(chǎn)品圖片
Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-ADFP (bs-1164R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kD
Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ADFP Polyclonal Antibody, Unconjugated(bs-1164R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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