| 產(chǎn)品編號(hào) | bs-0057R |
| 英文名稱 | Rabbit Anti-Amylin antibody |
| 中文名稱 | 糖尿病相關(guān)肽/胰島淀粉樣肽抗體 |
| 別 名 | Islet amyloid polypeptide; IAPP; DAP; Diabetes associated peptide; IAP; Insulinoma amyloid peptide. |
| 研究領(lǐng)域 | 心血管 信號(hào)轉(zhuǎn)導(dǎo) 糖尿病 新陳代謝 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Chicken,Dog) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 10kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Amylin: 1-37/89 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Amylin is commonly found in pancreatic islets of patients suffering diabetes mellitus type II, or harboring an insulinoma. While the assosciation of amylin with the development of type II diabetes has been known for some time, a direct causative role for amylin has been harder to establish. Recent results suggest that amylin, like the related beta amyloid (Abeta) assosciated with Alzheimer's disease, can induce apoptotic cell death in particular cultured cells, an effect that may be relevant to the development of type II diabetes.
Function: Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism. Subunit: Interacts with IDE and INS. Can form homodimers. Interaction with INS inhibits homodimerization and fibril formation. Subcellular Location: Secreted. Post-translational modifications: Amyloid fibrils are degraded by IDE. Similarity: Belongs to the calcitonin family. SWISS: P10997 Gene ID: 3375 Database links: Entrez Gene: 3375 Human Entrez Gene: 15874 Mouse Entrez Gene: 100009489 Rabbit Omim: 147940 Human SwissProt: P10997 Human SwissProt: P12968 Mouse SwissProt: Q07334 Rabbit Unigene: 46835 Human Unigene: 415 Mouse Unigene: 11394 Rat 胰島淀粉樣肽(islet amyloid polypeptide,IAPP)又稱amylin 或糖尿病相關(guān)肽是由胰腺β細(xì)胞產(chǎn)生的含37個(gè)氨基酸的一種短肽。IAPP降低胰島素的敏感性，抑制肌肉組織中葡萄糖的攝取和糖原的合成，抑制病人攝取食物。 |
| 產(chǎn)品圖片 |
Tissue/cell: Rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Amylin Polyclonal Antibody, Unconjugated(bs-0057R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Amylin) Polyclonal Antibody, Unconjugated (bs-0057R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Amylin Polyclonal Antibody, Unconjugated(bs-0057R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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