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Rabbit Anti-BNP  antibody (bs-0044R)  
~~~促銷(xiāo)代碼KT202411~~~
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產(chǎn)品編號(hào) bs-0044R
英文名稱(chēng) Rabbit Anti-BNP  antibody
中文名稱(chēng) 腦鈉素/利鈉肽抗體
別    名 BNP 32; Brain natriuretic peptide 32; Gamma brain natriuretic peptide; Natriuretic peptide brain type; Natriuretic peptide precursor B; Natriuretic peptides B; NPPB; NPPB protein; ANFB_HUMAN; Natriuretic peptides B.proBNP.  
研究領(lǐng)域 心血管  信號(hào)轉(zhuǎn)導(dǎo)  生長(zhǎng)因子和激素  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
交叉反應(yīng) Mouse,Rat (predicted: Human)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 3.5kDa
細(xì)胞定位 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human BNP 32: 103-120/134 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C-type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32 amino acid peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity


Function:
Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.

Subcellular Location:
Secreted.

Tissue Specificity:
Brain and also in atria, but at much lower levels than ANP.

Similarity:
Belongs to the natriuretic peptide family.

SWISS:
P16860

Gene ID:
4879

Database links:

Entrez Gene: 4879 Human

Omim: 600295 Human

SwissProt: P16860 Human

Unigene: 219140 Human



腦鈉素主要是心臟激素,心臟作為內(nèi)分泌器官釋放腦鈉素,調(diào)解體液及電解質(zhì)內(nèi)環(huán)境的恒定性。
B型利鈉肽(鈉尿肽)(B-typenatriureticpeptide,BNP),又稱(chēng)腦鈉素或腦鈉肽,因其有排鈉利尿、內(nèi)皮非依賴性的血管平滑肌作用而具有降壓、抗心肌纖維化、影響神經(jīng)內(nèi)分泌系統(tǒng)等功效。
近來(lái)發(fā)現(xiàn)BNP在各種心血管疾病,尤其是心室功能不全、急性心肌梗死(ami)、高血壓等疾病的診斷、治療及預(yù)后等方面有重要意義,已引起臨床工作者的高度重視。


產(chǎn)品圖片
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-BNP Polyclonal Antibody, Unconjugated(bs-0044R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-BNP Polyclonal Antibody, Unconjugated(bs-0044R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse kidney tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-BNP Polyclonal Antibody, Unconjugated(bs-0044R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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