| 產品編號 | bs-0095R |
| 英文名稱 | Rabbit Anti-CNN1 antibody |
| 中文名稱 | 鈣調節(jié)蛋白-1抗體 |
| 別 名 | CNN1_HUMAN; Calponin-1; Basic calponin; Calponin H1, smooth muscle; calponin 1; SMCC; Sm-Calp; HEL-S-14; |
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Specific References (12) | bs-0095R has been referenced in 12 publications.
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| 研究領域 | 心血管 信號轉導 干細胞 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Pig,Sheep,Cow,Chicken,Dog) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 33kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Calponin 1: 201-297/297 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Predicted to enable actin binding activity. Involved in negative regulation of vascular associated smooth muscle cell proliferation. Located in cytoskeleton. [provided by Alliance of Genome Resources, Nov 2021] Function: Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity). Subunit: Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1. Tissue Specificity: Smooth muscle, and tissues containing significant amounts of smooth muscle. Similarity: Belongs to the calponin family. Contains 3 calponin-like repeats. Contains 1 CH (calponin-homology) domain. SWISS: P51911 Gene ID: 1264 Database links: Entrez Gene: 1264 Human Entrez Gene: 12797 Mouse Omim: 600806 Human SwissProt: P51911 Human SwissProt: Q08091 Mouse Unigene: 465929 Human Unigene: 4356 Mouse Unigene: 31788 Rat Calponin1是一種激動蛋白結合蛋白，是平滑肌特有的一種蛋白，可以與肌動蛋白、原肌球蛋白和鈣調素結合,具有抑制平滑肌細胞收縮的功能，主要用于平滑肌腫瘤和乳腺等組織中的肌上皮細胞分布的研究。 |
| 產品圖片 |
Sample:
Urinary bladder (Mouse) Lysate at 40 ug
Primary: Anti-Calponin 1 (bs-0095R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD
Tissue/cell: mouse stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Calponin 1 Polyclonal Antibody, Unconjugated(bs-0095R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Calponin 1 Polyclonal Antibody, Unconjugated(bs-0095R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
