| 產(chǎn)品編號 | bs-0246R |
| 英文名稱 | Rabbit Anti-CRF antibody |
| 中文名稱 | 促腎上腺皮質(zhì)激素釋放因子/促腎上皮質(zhì)激素釋放激素抗體 |
| 別 名 | Corticoliberin; Corticoliberin precursor; Corticotropin releasing factor; Corticotropin releasing hormone; Corticotropin releasing hormone deficiency included; crf; crh; crh deficiency included; corticoliberin preproprotein; CRF_HUMAN; Corticotropin-releasing factor; CRH1; CRH; Corticotropin-releasing hormone. |
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Specific References (4) | bs-0246R has been referenced in 4 publications.
[IF=15.071] Balan et al. The GABAA Receptor α2 Subunit Activates a Neuronal TLR4 Signal in the Ventral Tegmental Area that Regulates Alcohol and Nicotine Abuse. (2018) Brain.Sci. 8 WB ; human.
[IF=6.208] Steven L. Bernstein. et al. Neuroprotection and Neuroregeneration Strategies Using the rNAION Model: Theory, Histology, Problems, Results and Analytical Approaches. INT J MOL SCI. 2022 Jan;23(24):15604 IHC ; Rat.
[IF=5.96] Balan, Irina, et al. "Innately activated TLR4 signal in the nucleus accumbens is sustained by CRF amplification loop and regulates impulsivity." Brain, Behavior, and Immunity(2017). WB, IF(ICC) ; Rat.
[IF=3.096] Roland Csurgyók. et al. Expression of Wilms-tumor 1 antigen, vimentin and corticotropin releasing factor in human kidney with focal segmental glomerulosclerosis and effect of oxidative stress on these markers in HEK 293 cells. KIDNEY BLOOD PRESS R. 2022 Dec;:1 IF, TEM ; Human.
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| 研究領(lǐng)域 | 神經(jīng)生物學(xué) 內(nèi)分泌病 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Rabbit,Pig,Chicken,Dog,GuineaPig) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 45434kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CRF: 185-196/196 |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Corticotropin-releasing hormone is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Marked reduction in this protein has been observed in association with Alzheimer disease and autosomal recessive hypothalamic corticotropin deficiency has multiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of the hormone occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, this protein may act as a trigger for parturition. [provided by RefSeq, Apr 2010]. Function: This hormone from hypothalamus regulates the release of corticotropin from pituitary gland. Subunit: Interacts (via C-terminus) with CRFR1 (via N-terminalv extracellular domain). Subcellular Location: Secreted. Similarity: Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family. SWISS: P06850 Gene ID: 1392 Database links: Entrez Gene: 1392 Human Entrez Gene: 12918 Mouse Omim: 122560 Human SwissProt: P06850 Human SwissProt: Q8CIT0 Mouse Unigene: 75294 Human Unigene: 290689 Mouse Unigene: 10349 Rat CRF陽性神經(jīng)元的胞體廣泛分布于下丘腦的室旁核、背縫核和藍(lán)斑核，這些區(qū)域也是去甲腎上腺素（NE）神經(jīng)元與5－羥色胺（5－HT）神經(jīng)元密集的區(qū)域。 CRF既具有神經(jīng)遞質(zhì)的性質(zhì)又具有神經(jīng)激素的性質(zhì)。CRF是聯(lián)系神經(jīng)系統(tǒng)與內(nèi)分泌系統(tǒng)的橋梁，在情感障礙疾患如焦慮和抑郁的發(fā)病中發(fā)揮重要作用. |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRF) Polyclonal Antibody, Unconjugated (bs-0246R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRF) Polyclonal Antibody, Unconjugated (bs-0246R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRF) Polyclonal Antibody, Unconjugated (bs-0246R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRF) Polyclonal Antibody, Unconjugated (bs-0246R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRF) Polyclonal Antibody, Unconjugated (bs-0246R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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