| 產(chǎn)品編號 | bs-0456R |
| 英文名稱 | Rabbit Anti-GNRH/LHRH antibody |
| 中文名稱 | 黃體激素釋放激素類似物抗體 |
| 別 名 | GNRH 1; GnRH associated peptide 1; GNRH1; Gonadotrophin Releasing Hormone 1; GRH; HH12; GON1_HUMAN; LHRH; LNRH; Luliberin I; Luteinizing releasing hormone; Lutenizing Hormome Releasing Hormone; Progonadoliberin 1; Progonadoliberin I; Progonadoliberin1; ProgonadoliberinI; Prolactin Release Inhibiting Factor; Prolctin. |
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Specific References (2) | bs-0456R has been referenced in 2 publications.
[IF=4.57] Liu, Hongxia, et al. "Ultrasound-Mediated Destruction of LHRHa Targeted and Paclitaxel Loaded Lipid Microbubbles Induces Proliferation Inhibition and Apoptosis in Ovarian Cancer Cells." Molecular Pharmaceutics (2013). ICF ; Human.
[IF=1.514] Liu T et al. Di-(2-ethylhexyl) phthalate induces precocious puberty in adolescent female rats.Iran J Basic Med Sci.?2018 Aug;21(8):848-855. IHC-P ; Rat.
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| 研究領(lǐng)域 | 腫瘤 神經(jīng)生物學(xué) 生長因子和激素 內(nèi)分泌病 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 7.9kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide ([D-Trp6]-LHRH Fragment, 1-6): 28-40/92 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is secreted and then cleaved to form the 10 aa luteinizing hormone-releasing hormone (LHRH, also known as gonadoliberin-1), and prolactin release-inhibiting factor (also known as GnRH-associated peptide 1). LHRH stimulates the release of luteinizing and follicle stimulating hormones, which are important for reproduction. Mutation in this gene are associated with hypogonadotropic hypogonadism. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2012]. Function: Stimulates the secretion of gonadotropins; it stimulates the secretion of both luteinizing and follicle-stimulating hormones. Subcellular Location: Secreted. DISEASE: Hypogonadotropic hypogonadism 12 with or without anosmia (HH12) [MIM:614841]: A disorder characterized by absent or incomplete sexual maturation by the age of 18 years, in conjunction with low levels of circulating gonadotropins and testosterone and no other abnormalities of the hypothalamic-pituitary axis. In some cases, it is associated with non-reproductive phenotypes, such as anosmia, cleft palate, and sensorineural hearing loss. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In the presence of anosmia, idiopathic hypogonadotropic hypogonadism is referred to as Kallmann syndrome, whereas in the presence of a normal sense of smell, it has been termed normosmic idiopathic hypogonadotropic hypogonadism (nIHH). Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the GnRH family. SWISS: P01148 Gene ID: 2796 Database links:
Entrez Gene: 2796 Human Entrez Gene: 14714 Mouse Omim: 152760 Human SwissProt: P01148 Human SwissProt: P13562 Mouse SwissProt: Q28588 Sheep
生長因子和激素（ Growth Factor and Hormones） ＬＨＲＨ拮抗劑可以競爭性結(jié)合垂體ＬＨＲＨ的受體，而使天然的ＬＨＲＨ不能發(fā)揮作用，進而抑制垂體的促性腺激素的分泌，起到藥物性去垂體的作用，睪丸失去了促性腺激素的刺激作用，分泌雄激素和產(chǎn)生精子的功能隨之受到抑制，實驗證明ＬＨＲＨ拮抗劑同樣是一種有前途的抗精子生成藥物。 應(yīng)用ＬＨＲＨ抗體可用來研究癡呆和神經(jīng)變性疾病、不孕不育、某些腫瘤方面的研究。 ＬＨＲＨ類似物作為一種男性避孕藥的優(yōu)點是沒有生殖系統(tǒng)以外作用，無毒副作用，而且抗生育作用是可逆的 促性腺激素釋放激素抗體 |
| 產(chǎn)品圖片 |
Tissue/cell: human mammary cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GNRH Polyclonal Antibody, Unconjugated(bs-0456R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (human kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GNRH) Polyclonal Antibody, Unconjugated (bs-0456R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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