| 產(chǎn)品編號 | bs-0642R |
| 英文名稱 | Rabbit Anti-MAdCAM-1 antibody |
| 中文名稱 | 粘膜血管定居因子抗體 |
| 別 名 | Mucosal addressin cellular adhesion molecule-1; Addressin mucosal; hMAdCAM 1; MACAM1; Mucosal addressin cell adhesion molecule 1; Mucosal vascular addressin cell adhesion molecule 1; MADCA_RAT. |
| 研究領(lǐng)域 | 腫瘤 心血管 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 40kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from rat MAdCAM-1: 151-250/394 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008] Function: Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both the integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes (By similarity). Subunit: Homodimer (Probable). Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Detected in Peyer patches and mesenteric lymph nodes but not in spleen. Similarity: Contains 3 Ig-like (immunoglobulin-like) domains. SWISS: O70540 Gene ID: 54266 Database links: Entrez Gene: 17123 Mouse SwissProt: Q61826 Mouse Unigene: 391556 Mouse MAdCAM-1（粘膜血管定居因子或黏膜地址素細(xì)胞黏附分子）、CD44等均參與癌癥和腫瘤細(xì)胞與血管內(nèi)皮的相互作用，與癌癥和腫瘤細(xì)胞一起穿過血管內(nèi)皮而進入血管內(nèi)，參與血行播散轉(zhuǎn)移。 |
| 產(chǎn)品圖片 |
Sample:
Lymph node (Mouse) Lysate at 30 ug
Spleen (Mouse) Lysate at 30 ug
Primary: Anti- MAdCAM-1 (bs-0642R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 50 kD
Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-MAdCAM-1 Polyclonal Antibody, Unconjugated(bs-0642R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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