| 產品編號 | bs-0557R |
| 英文名稱 | Rabbit Anti-Mitofusin 1 antibody |
| 中文名稱 | 線粒體融合蛋白1抗體 |
| 別 名 | Mitofusin 1; Fzo homolog; MFN 1; Mitochondrial transmembrane GTPase Fzo 1; Mitochondrial transmembrane GTPase FZO 2; Mitochondrial transmembrane GTPase FZO1B; Mitofusin 1; Mitofusin1; Putative transmembrane GTPase; Transmembrane GTPase MFN1; MFN1_HUMAN; Mitofusin-1. |
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Specific References (9) | bs-0557R has been referenced in 9 publications.
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| 研究領域 | 腫瘤 細胞生物 神經生物學 信號轉導 細胞凋亡 新陳代謝 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Human,Mouse,Rabbit,Horse) |
| 產品應用 | Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 81kDa |
| 細胞定位 | 細胞漿 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Mfn 1: 651-741/741 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Mitofusin 1 (Mfn1) and mitofusin 2 (Mfn2) are homologs for the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins and are mediators of mitochondrial fusion. A GTPase domain is required for Mfn protein function but the molecular mechanisms of the GTPase-dependent reaction as well as the functional division of the two Mfn proteins are unknown. They are essential for embryonic development and may play a role in the pathobiology of obesity. Although the Mfn1 and Mfn2 genes are broadly expressed, they show different levels of expression in different tissues. Two Mfn1 transcripts are elevated in heart, while Mfn2 mRNA is abundantly expressed in heart and muscle tissue but present only at low levels in many other tissues. Mfn1 localizes to mitochondria and participates in at least two different high molecular weight protein complexes in a GTP-dependent manner. Purified recombinant Mfn1 exhibited approximately eightfold higher GTPase activity than Mfn2. Function: Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks. Subunit: Forms homomultimers and heteromultimers with MFN2. Multimerization, which is mediated by the second coiled coil region, may play an essential role in mitochondrion fusion. Participates in a high molecular weight multiprotein complex. Interacts with VAT1. Subcellular Location: Cytoplasm and Mitochondrion outer membrane. Tissue Specificity: Ubiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers. Post-translational modifications: Ubiquitinated by MARCH5. Similarity: Belongs to the mitofusin family. SWISS: Q8IWA4 Gene ID: 55669 Database links: Entrez Gene: 55669 Human Entrez Gene: 67414 Mouse Omim: 608506 Human SwissProt: Q8IWA4 Human SwissProt: Q811U4 Mouse Unigene: 478383 Human Unigene: 290414 Mouse Unigene: 160939 Rat 線粒體融合蛋白1具有促進線粒體融合、抑制細胞增殖、保護細胞免于凋亡等多種功能。 |
| 產品圖片 |
Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue),
Dilution: 1:200 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
