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Rabbit Anti-Nociceptin  antibody (bs-0075R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號(hào) bs-0075R
英文名稱 Rabbit Anti-Nociceptin  antibody
中文名稱 孤菲肽/痛敏肽抗體
別    名 PNOC; N/OFQ; N23K; N23K/N27K; Nociceptin, included; Npnc1; OFQ; OFQ/N; Orphanin FQ; PPNOC; prepronociceptin; Propronociceptin; PNOC_HUMAN.  
Specific References  (1)     |     bs-0075R has been referenced in 1 publications.
[IF=1.931] Lyu J et al. Botulinum toxin A alleviates orofacial nociception induced by orthodontic tooth movement through nociceptin/orphanin-FQ pathway in rats. Arch Oral Biol . 2020 Sep;117:104817.  WB ;  Rat.  
研究領(lǐng)域 腫瘤  心血管  神經(jīng)生物學(xué)  信號(hào)轉(zhuǎn)導(dǎo)  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Rat (predicted: Human,Mouse,Rabbit,Pig,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 20kDa
細(xì)胞定位 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Nociceptin: 130-146/176 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Nociceptin (PNOC) is the ligand of the opioid receptor-like receptor (OPRL1). It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development. Expressed predominantly in the spinal cord and brain, being more abundant in the hypothalamus and striatum. Also found in small amounts in ovary. Belongs to the opioid neuropeptide precursor family.


Function:
Nociceptin is the ligand of the opioid receptor-like receptor (OPRL1). It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development (By similarity).

Subcellular Location:
Secreted.

Tissue Specificity:
Predominantly in the brain and spinal cord.

Similarity:
Belongs to the opioid neuropeptide precursor family.

SWISS:
Q13519

Gene ID:
5368

Database links:

Entrez Gene: 5368 Human

Entrez Gene: 18155 Mouse

Entrez Gene: 25516 Rat

Omim: 601459 Human

SwissProt: Q13519 Human

SwissProt: Q64387 Mouse

SwissProt: Q62923 Rat

Unigene: 88218 Human

Unigene: 16347 Mouse

Unigene: 486163 Mouse

Unigene: 87935 Rat



孤菲肽(Nociceptin又稱為Orphanin FQ; PPNOC; ORL1)是新發(fā)現(xiàn)的一種神經(jīng)肽,由17個(gè)氨基酸組成,又稱痛敏肽。消化道腫瘤病人的血漿中OFQ含量明顯高于對(duì)照組,尤以結(jié)腸癌、胃癌最為明顯。
產(chǎn)品圖片
Sample: Brain(Rat) lysate at 30ug; Liver(Rat) lysate at 30ug; Primary: Anti-Nociceptin(bs-0075R) at 1:200 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 20kD Observed band size : 20kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Nociceptin Polyclonal Antibody, Unconjugated(bs-0075R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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