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Rabbit Anti-PDGF-A  antibody (bs-0196R)  
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產(chǎn)品編號 bs-0196R
英文名稱 Rabbit Anti-PDGF-A  antibody
中文名稱 血小板源性生長因子A抗體
別    名 Platelet–dirived growth factor–1; PDGFA; PDGF AA; PDGF-AA; PDGF A chain; PDGF subunit A; PDGF-1; PDGF1; PDGFA; PDGFA_HUMAN; Platelet derived growth factor A chain precursor; Platelet derived growth factor alpha polypeptide; Platelet-derived growth factor A chain; Platelet-derived growth factor alpha polypeptide; Platelet-derived growth factor subunit A.  
Specific References  (7)     |     bs-0196R has been referenced in 7 publications.
[IF=16.036] Valentina Back. et al. Inhibition of platelet aggregation by activation of platelet intermediate conductance Ca2+-activated potassium channels. J THROMB HAEMOST. 2022 Aug;:  WB ;  Human.  
[IF=6.064] Yu Hedong. et al. Improved repair of rabbit calvarial defects with hydroxyapatite/chitosan/polycaprolactone composite scaffold-engrafted EPCs and BMSCs. FRONT BIOENG BIOTECH. 2022 Aug;0:1275  WB ;  Rabbit.  
[IF=5.097] Yan L et al. Exosomes produced from 3D cultures of umbilical cord mesenchymal stem cells in a hollow-fiber bioreactor show improved osteochondral regeneration activity. Cell Biology and Toxicology.  WB ;  Human.  
[IF=4.075] Wang et al. A Bayesian Framework for Generalized Linear Mixed Modeling Identifies New Candidate Loci for Late-Onset Alzheimer's Disease. (2018) Genetics. 209:51-64  IF ;  Mouse.  
[IF=2.77] Lee, Si‐Hyung, et al. "Therapeutic efficacy of autologous platelet‐rich plasma and polydeoxyribonucleotide on female pattern hair loss." Wound Repair and Regeneration (2014).  WB ;  
[IF=2.583] Du?L et al. A Novel and Convenient Method for the Preparation and Activation of PRP without Any Additives: Temperature Controlled PRP. Biomed Res Int. 2018 May 13;2018:1761865.  WB ;  Human.  
[IF=1.64] Eren, Kenan, et al. "The Suppression of Wound Healing Response with Sirolimus and Sunitinib Following Experimental Trabeculectomy in a Rabbit Model." Current Eye Research (2016): 1-10.  IHC-P ;  Rabbit.  
研究領(lǐng)域 腫瘤  免疫學  神經(jīng)生物學  生長因子和激素  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse (predicted: Rat,Rabbit)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=0.2μg/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 24kDa
細胞定位 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from the missle of human PDGF-A: 125-170/211 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heal the wound. Antiparallel disulfide-linked dimer of nonidentical (A and B) chains. Homodimers of A and B chains are implicated in transformation processes. The long form contains a basic insert which acts as a cell retention signal. A-A and B-B, as well as A-B, dimers can bind to the PDGF receptor. It belongs to the PDGF/VEGF growth factor family.

Function:
Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB.

Subunit:
Homodimer; antiparallel disulfide-linked dimer. Heterodimer with PDGFB; antiparallel disulfide-linked dimer. The PDGFA homodimer interacts with PDGFRA homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PDGFA and PDGFB interacts with PDGFRA homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts with CSPG4.

Subcellular Location:
Secreted. Note=Released by platelets upon wounding.

Tissue Specificity:
Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.

Similarity:
Belongs to the PDGF/VEGF growth factor family.

SWISS:
P04085

Gene ID:
5154

Database links:

Entrez Gene: 5154 Human

Omim: 173430 Human

SwissProt: P04085 Human

Unigene: 535898 Human



PDGF-A(platelet-dirived growth factor-A)是一種堿性蛋白,pdgf促進細胞分裂、增值同時,可誘導c-fos、c-myc等蛋白表達增強。
主要作用:參與組織和血管修復、促進平滑肌細胞、成纖維細胞迅速增值。分子量為28-35kDa,分為兩個亞基(A、B鏈)通過二硫鍵結(jié)合而成的兩條多肽鏈的異二聚體。最新研究發(fā)現(xiàn)了PDGF家族兩種新的基因PDGF-C、PDGF-D。
產(chǎn)品圖片
Sample: A549 (human)Cell Lysate at 40 ug Primary: Anti- PDGF-A (bs-0196R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 14 kD Observed band size: 18 kD
Sample: placenta (Mouse) Lysate at 40 ug Primary: Anti-PDGF-A (bs-0196R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 14 kD Observed band size: 17 kD
Blank control (blue line): A431 cells (blue). Primary Antibody (green line): Rabbit Anti-PDGF-A antibody (bs-0196R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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