| 產(chǎn)品編號(hào) | bs-0250R |
| 英文名稱 | Rabbit Anti-PPAR delta + beta antibody |
| 中文名稱 | D型-過氧化酶活化增生受體抗體 |
| 別 名 | PPAR delta+beta; FAAR; MGC3931; NR1C2; NUC1; NUCI; NUCII; Nuclear hormone receptor 1; Nuclear receptor subfamily 1 group C member 2; Peroxisome proliferative activated receptor delta; Peroxisome proliferator-activated receptor beta (PPAR-beta); Peroxisome proliferator-activated receptor beta; Peroxisome proliferator-activated receptor delta; PPAR beta; PPAR-beta; PPAR-delta; PPAR-? PPARB; PPARD; PPARD_HUMAN. |
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Specific References (1) | bs-0250R has been referenced in 1 publications.
[IF=4.398] Xiaoyu Qu. et al. Integration of metabolomics and proteomics analysis to explore the mechanism of neurotoxicity induced by receipt of isoniazid and rifampicin in mice. NEUROTOXICOLOGY. 2023 Jan;94:24 IHC ; Mouse.
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| 研究領(lǐng)域 | 心血管 免疫學(xué) 細(xì)胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1ug/Test,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 48kDa |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse PPAR-delta: 2-100/440 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Heterodimer with the retinoid X receptor. Subcellular located at nuclear Tissue specificity: Heart, adrenal and intestine. Belongs to the nuclear hormone receptor family. NR1 subfamily. It Contains 1 nuclear receptor DNA-binding domain. Function: Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand. Subcellular Location: Nucleus. Tissue Specificity: Ubiquitous with maximal levels in placenta and skeletal muscle. Similarity: Belongs to the nuclear hormone receptor family. NR1 subfamily. Contains 1 nuclear receptor DNA-binding domain. SWISS: P35396 Gene ID: 5467 Database links: Entrez Gene: 5467 Human Entrez Gene: 19015 Mouse Omim: 600409 Human SwissProt: Q03181 Human SwissProt: P35396 Mouse Unigene: 696032 Human Unigene: 328914 Mouse Unigene: 96181 Rat 類固醇受體（Steroid Receptors） 研究人員指出該分子的功能，極可能就是阻斷心血管免疫發(fā)炎反應(yīng)的訊號(hào)傳遞過程。PPAR－delta基因是控制肌肉發(fā)展的重要基因，在增加老鼠耐力的同時(shí)還能幫助它燃燒掉脂肪，研究人員發(fā)現(xiàn)，對(duì)于與新陳代謝有關(guān)的各項(xiàng)疾病，從心臟病到肥胖癥都有啟發(fā)性的意義，他可以阻止脂肪沉淀在動(dòng)脈壁上，進(jìn)而防止動(dòng)脈硬化癥的發(fā)生,該蛋白參與脂肪代謝、肥胖、糖尿病、動(dòng)脈硬化和癌癥的發(fā)病。 在動(dòng)脈硬化現(xiàn)象發(fā)生的早期，免疫細(xì)胞會(huì)促使血管慢性的發(fā)炎，因而造成血管不斷地吸收和輸送脂肪，進(jìn)而導(dǎo)致脂肪的堆積與血管硬化，而PPAR-delta就像警衛(wèi)分子一樣，時(shí)時(shí)降低發(fā)炎反應(yīng)和抑制動(dòng)脈硬化斑的形成，因此該分子應(yīng)該是相關(guān)藥物研發(fā)，相當(dāng)值得切入的目標(biāo)。 研究人員認(rèn)為，這個(gè)發(fā)現(xiàn)將給藥廠研發(fā)治療心血管疾病的新藥提供新的線索。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Placenta tissue lysates
Lane 2: Mouse Testis tissue lysates
Primary: Anti-PPAR delta + beta (bs-0250R) at 1/200 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 53 kDa
Tissue/cell: Human esophageal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PPAR-delta Polyclonal Antibody, Unconjugated(bs-0250R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-PPAR delta + beta antibody (bs-0250R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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