| 產品編號 | bs-1331R |
| 英文名稱 | Rabbit Anti-SFRP4 antibody |
| 中文名稱 | 子宮內膜抗體 |
| 別 名 | sFRP-4; Secreted frizzled-related protein 4; Frizzled protein, human endometrium; FrpHE; SFRP4; SFRP4_MOUSE; SFRP4_HUMAN; FRPHE. |
| 研究領域 | 免疫學 干細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Dog) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 38kDa |
| 細胞定位 | 細胞漿 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human sFRP-4: 151-260/351 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake. [SUBCELLULAR LOCATION] Secreted. Expressed in mesenchymal cells. Highly expressed in the stroma of proliferative endometrium. Expressed in cardiomyocytes. Belongs to the secreted frizzled-related protein (sFRP) family. Function: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling. Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake (By similarity). Subcellular Location: Secreted. Tissue Specificity: Expressed the ovary. Localized to granulosa cells of periovulatory follicles and corpora lutea. Weakly expressed in adult tissues including kidney, brain and lung. Similarity: Belongs to the secreted frizzled-related protein (sFRP) family. Contains 1 FZ (frizzled) domain. Contains 1 NTR domain. SWISS: Q6FHJ7 Gene ID: 6424 Database links: Entrez Gene: 6424 Human Entrez Gene: 20379 Mouse Omim: 606570 Human SwissProt: Q6FHJ7 Human SwissProt: Q9Z1N6 Mouse Unigene: 658169 Human Unigene: 42095 Mouse Unigene: 10788 Rat 抗子宮內膜抗體屬于自身抗體，在正常育齡婦女中也可以檢測到，但在不孕癥人群中，特別是患有子宮內膜異位癥的婦女中更多見。 正常機體具有自身免疫調節(jié)功能，產生極弱的自身抗體，幫助清除體內衰老變性的自身成分，但由于某種原因導致免疫系統對自身組織產生過度免疫應答，則會發(fā)生過強的一系列免疫反應，致使所侵及的組織免疫活性細胞增多，免疫復合物沉積，而導致功能改變。當這種抗子宮內膜抗體由于反復刺激而大量產生達到一定的含量時，可與自身的子宮內膜組織發(fā)生抗原抗體結合反應，并激活免疫系統引起損傷性效應，造成子宮內膜組織細胞生化代謝及生理功能的損害，干擾和妨礙精卵結合，受精卵的著床和胚囊的發(fā)育而導致不孕或流產。 |
| 產品圖片 |
Sample:
Bone (Mouse) Lysate at 40 ug
Primary: Anti-SFRP4 (bs-1331R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 60 kD
Tissue/cell: human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-sFRP-4 Polyclonal Antibody, Unconjugated(bs-1331R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
