| 產(chǎn)品編號(hào) | bs-1316R |
| 英文名稱(chēng) | Rabbit Anti-PDGFBB antibody |
| 中文名稱(chēng) | 血小板源性生長(zhǎng)因子BB抗體 |
| 別 名 | PDGF BB; PDGF-BB; platelet derived growth factor-BB; Becaplermin; C sis; FLJ12858; Oncogene SIS; PDG B chain; PDGB; PDGF 2; PDGF B; PDGF B chain; PDGF2; PDGFB; PDGFB/COL1A1 fusion gene; Platelet derived growth factor 2; Platelet derived growth factor B chain; Platelet derived growth factor beta; Platelet derived growth factor beta polypeptide; Simian sarcoma viral (v sis) oncogene homolog; SIS; SSV; V sis platelet derived growth factor beta polypeptide. |
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Specific References (5) | bs-1316R has been referenced in 5 publications.
[IF=14.511] Ying Xia. et al. SB431542 alleviates lupus nephritis by regulating B cells and inhibiting the TLR9/TGFβ1/PDGFB signaling. J AUTOIMMUN. 2022 Oct;132:102894 IHC ; Mouse.
[IF=13.116] Yan Li. et al. Injectable hydrogel with MSNs/microRNA-21-5p delivery enables both immunomodification and enhanced angiogenesis for myocardial infarction therapy in pigs. Sci Adv. 2021 Feb;7(9):eabd6740 WB ; Pig.
[IF=10.171] Qi Wu. et al. Glut10 restrains neointima formation by promoting SMCs mtDNA demethylation and improving mitochondrial function. TRANSL RES. 2023 May;: IHC ; Rat，Human.
[IF=4.897] Tang L et al. RhoA/ROCK signaling regulates smooth muscle phenotypic modulation and vascular remodeling via the JNK pathway and vimentin cytoskeleton.Pharmacol Res. 2018 Jul;133:201-212. IF&WB ; Human.
[IF=1.97] Cipriani et al. Searching for a good model for systemic sclerosis: the molecular profile and vascular changes occurring in UCD-200 chickens strongly resemble the early phase of human systemic sclerosis. (2016) Arch.Med.Sci. 12:828-43 IF ; Chicken.
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| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 12kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PDGF-B: 151-241/241 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Platelet Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimer composed of two related polypeptides designated A and B, the dimers being connected by disulfide bonds. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis. Function: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA. Subunit: Homodimer; antiparallel disulfide-linked dimer. Heterodimer with PDGFA; antiparallel disulfide-linked dimer. The PDGFB homodimer interacts with PDGFRA and PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PDGFA and PDGFB interacts with PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts with XLKD1. Subcellular Location: Secreted. Note=Released by platelets upon wounding. Tissue Specificity: Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung. DISEASE: Note=A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB. Similarity: Belongs to the PDGF/VEGF growth factor family. SWISS: P01127 Gene ID: 5155 Database links: Entrez Gene: 5155 Human Entrez Gene: 18591 Mouse Omim: 190040 Human SwissProt: P01127 Human SwissProt: P31240 Mouse Unigene: 1976 Human Unigene: 144089 Mouse Unigene: 198230 Rat 生長(zhǎng)因子和激素（ Growth Factor and Hormones） 血小板源性生長(zhǎng)因子（PDGF），是由A、B兩條多肽鏈通過(guò)二硫鍵連接而成的同型或異型二聚體。包括三種形式：PDGF-AA、PDGF-BB、PDGF-AB.分子量為28～35KD。 PDGF-BB也是PDGF家族成員，是一種堿性蛋白，可促進(jìn)細(xì)胞分裂并促進(jìn)神經(jīng)元的生長(zhǎng)， PDGF-BB的正常表達(dá)對(duì)于神經(jīng)系統(tǒng)的發(fā)育和成熟起著一定的作用。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Huvec (Human) Cell Lysate at 30 ug
Lane 2: A431 (Human) Cell Lysate at 30 ug
Lane 3: MCF-7 (Human) Cell Lysate at 30 ug
Primary:
Anti-PDGFBB (bs-1316R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27/22/14 kD
Observed band size: 30 kD
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PDGF-BB Polyclonal Antibody, Unconjugated(bs-1316R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PDGF-BB Polyclonal Antibody, Unconjugated(bs-1316R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PDGFBB) Polyclonal Antibody, Unconjugated (bs-1316R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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