| 產品編號 | bs-0924R |
| 英文名稱 | Rabbit Anti-Mafa antibody |
| 中文名稱 | v-maf 肌腱膜纖維肉瘤癌基因同源物A抗體 |
| 別 名 | Mafa homolog; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A; Pancreatic beta-cell-specific transcriptional activator; (avian)(V-maf musculoaponeurotic fibrosarcoma oncogene homolog A; MAFA_MOUSE. |
| 研究領域 | 神經生物學 信號轉導 轉錄調節(jié)因子 糖尿病 內分泌病 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Human,Mouse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 37kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse Mafa: 265-359/359 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Insulin gene expression is regulated by several islet-enriched transcription factors. However, MAFA is the only beta cell-specific activator. MAFA selectively induces endogenous insulin transcription in non-beta cells. MAFA was also first detected in the insulin-producing cells formed during the second and predominant phase of beta cell differentiation, and absent in the few insulin-positive cells found in Nkx6.1(-/-) pancreata, which lack the majority of second-phase beta cells. These results demonstrate that MAFA is a potent insulin activator that is likely to function downstream of Nkx6.1 during islet insulin-producing cell development. Function: Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Regulates the insulin gene transcription. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Subunit: Binds DNA as a homodimer. Interacts with PCAF. Interacts with NEUROD1 and PDX1. Subcellular Location: Nucleus. Note=Detected in nuclei of pancreas islet beta cells. Tissue Specificity: Selectively expressed in pancreatic beta but not in alpha cells (at protein level). Expressed in eyes and at low levels in thymus. Expressed in brain, lung, spleen and kidney. Expressed in embryo. Post-translational modifications: Ubiquitinated, leading to its degradation by the proteasome. Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation through an ubiquitin/proteasome-dependent pathway. Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable). Similarity: Belongs to the bZIP family. Maf subfamily. Contains 1 bZIP (basic-leucine zipper) domain. SWISS: Q8CF90 Gene ID: 378435 Database links: Entrez Gene: 389692 Human Entrez Gene: 378435 Mouse Omim: 610303 Human SwissProt: Q8NHW3 Human SwissProt: Q8CF90 Mouse Unigene: 521914 Human Unigene: 309589 Mouse |
| 產品圖片 |
Sample:
Lane 1: Rat Pancreas tissue lysates
Primary: Anti- Mafa (bs-0924R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 47 kDa
Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Mafa Polyclonal Antibody, Unconjugated(bs-0924R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
