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[IF=4.522] Elsaeid Elnour I et al. Bta‐miR‐885 promotes proliferation and inhibits differentiation of myoblasts by targeting MyoD1. J Cell Physiol. 2020 Jan 27.  WB ;  Bovine.  

PubMed:31985035

[IF=1.422] Ran M et al. miR-26a suppresses autophagy in swine Sertoli cells by targeting ULK2.Reprod Domest Anim. 2018 Aug;53(4):864-871.  WB ;  Pig.  

PubMed:29761550

Sample: K562 Cell Lysate at 30 ug Primary: Anti- Cdc6 (bs-1390R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63 kD Observed band size: 63 kD

Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cdc6) Polyclonal Antibody, Unconjugated (bs-1390R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: rat uterus tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cdc6 Polyclonal Antibody, Unconjugated(bs-1390R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Cdc6) polyclonal Antibody, Unconjugated (bs-1390R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Blank control:Hela. Primary Antibody (green line): Rabbit Anti-Cdc6 antibody (bs-1390R) Dilution: 1ug/Test; Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.