| 產(chǎn)品編號 | bs-1195R |
| 英文名稱 | Rabbit Anti-beta endorphin antibody |
| 中文名稱 | β-內(nèi)啡肽/β endorphin抗體 |
| 別 名 | β-Endorphin; β endorphin; βendorphin; Beta-endorphin; Corticotropin lipotropin; POMC; Proopiomelanocortin; COLI_HUMAN; β-EP; β EP; βEP. β內(nèi)啡肽; β 內(nèi)啡肽; |
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Specific References (1) | bs-1195R has been referenced in 1 publications.
[IF=3.73] Nakamoto, Kazuo, et al. ?Hypothalamic GPR40 Signaling Activated by Free Long Chain Fatty Acids Suppresses CFA-Induced Inflammatory Chronic Pain.? PLOS ONE 8.12 (2013): e81563. IHC-F ; Mouse.
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| 研究領(lǐng)域 | 免疫學(xué) 神經(jīng)生物學(xué) 內(nèi)分泌病 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Sheep,Cow) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 4.5kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ACTH: 237-267/267 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]. Function: ACTH stimulates the adrenal glands to release cortisol. MSH (melanocyte-stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes. Beta-endorphin and Met-enkephalin are endogenous opiates. Subcellular Location: Secreted. Tissue Specificity: ACTH and MSH are produced by the pituitary gland. Post-translational modifications: Specific enzymatic cleavages at paired basic residues yield the different active peptides. O-glycosylated; reducing sugar is probably N-acetylgalactosamine. DISEASE: Defects in POMC may be associated with susceptibility to obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD) [MIM:609734]. Affected individuals present early-onset obesity, adrenal insufficiency and red hair. Similarity: Belongs to the POMC family. SWISS: P01189 Gene ID: 5443 Database links: Entrez Gene: 5443 Human Omim: 176830 Human SwissProt: P01189 Human Unigene: 1897 Human 內(nèi)啡肽是體內(nèi)自己產(chǎn)生的一類內(nèi)源性的具有類似嗎啡作用的肽類物質(zhì)，是內(nèi)啡肽(endogenous)和嗎啡(morphine)的縮些。 內(nèi)啡肽（endorphin）具有嗎啡樣活性的神經(jīng)肽的總稱亦稱安多芬或腦內(nèi)啡，是由腦垂體分泌的類嗎啡生物化學(xué)合成物激素。它是由腦下垂體和脊椎動物的丘腦下部所分泌的氨基化合物(肽) 。它能與嗎啡受體結(jié)合，產(chǎn)生跟嗎啡、鴉片劑一樣有止痛和欣快感。等同天然的鎮(zhèn)痛劑。利用藥物可增加腦內(nèi)啡的分泌效果。 英文Endorphin是endomorphin的簡化寫法，endo有內(nèi)在之含意，而morphin則為嗎啡的英文名稱，故endorphin有大腦自我制造的類嗎啡物質(zhì)之意。 內(nèi)啡肽是體內(nèi)自己產(chǎn)生的一類內(nèi)源性的具有類似嗎啡作用肽類物質(zhì)。是內(nèi)源(endogenous)和嗎啡(morphine)的縮略詞。內(nèi)啡肽可包括α-內(nèi)啡肽、β-內(nèi)啡肽、γ-內(nèi)啡肽、蛋氨酸-腦啡肽、亮氨酸-腦啡肽、強啡肽A、強啡肽B等，都具有很強的類嗎啡活性。蛋氨酸-腦啡肽的氨基酸序列為酪氨酸-甘氨酸-甘氨酸-苯丙氨酸-蛋氨酸。亮氨酸-腦啡肽第五個氨基酸不是蛋氨酸而是亮氨酸。α、β、γ-內(nèi)啡肽分別為11肽、31肽、18肽，它們的前五個氨基酸序列與蛋氨酸-腦啡肽的五肽相同。這類肽具有很強的生理功能。向動物腦室中注射內(nèi)啡肽，可引起全身深度失去痛覺，體溫下降，行為變得木僵。再施以嗎啡拮抗劑納洛酮(naloxone)，不再有上述失痛感覺。內(nèi)啡肽誘導(dǎo)出的行為表明，這些肽可能參與感情應(yīng)答的調(diào)節(jié)從垂體中分離出的內(nèi)啡肽,其代表為β－內(nèi)啡肽及鎮(zhèn)痛作用更強的強啡肽。它們都屬于內(nèi)源性阿片肽，是機(jī)體抗痛系統(tǒng)的組成部分，具有生理意義。當(dāng)機(jī)體有傷痛刺激時，內(nèi)源性阿片肽被釋放出來以對抗疼痛。在內(nèi)腓肽的激發(fā)下，人的身心處于輕松愉悅的狀態(tài)中，免疫系統(tǒng)實力得以強化，并能順利入夢，消除失眠癥。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse pituitary gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (beta endorphin) Polyclonal Antibody, Unconjugated (bs-1195R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: mouse lymphoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-beta endorphin Polyclonal Antibody, Unconjugated(bs-1195R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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