| 產品編號 | bs-1456R |
| 英文名稱 | Rabbit Anti-Adenosine Receptor A2a antibody |
| 中文名稱 | 腺苷A2A受體抗體 |
| 別 名 | Adenosine Receptor A2a; Adenosine A2A-R; A2AR; A2A adenosine receptor; A2AAR; A2aR; ADENO; Adenosine A2 receptor; Adenosine A2a receptor; Adenosine receptor subtype A2a; Adenosine Receptor A2a; ADORA 2; ADORA2; Adora2a; hA2aR; RDC 8; RDC8; AA2AR_HUMAN. |
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Specific References (3) | bs-1456R has been referenced in 3 publications.
[IF=5.195] Junnan Cai. et al. Shufeng Jiedu capsule inhibits inflammation and apoptosis by activating A2AAR and inhibiting NF-κB to alleviate LPS-induced ALI. J ETHNOPHARMACOL. 2022 Nov;298:115661 IF, WB ; Mouse.
[IF=4.366] Aleix Martí Naviaet al. Adenosine Receptors as Neuroinflammation Modulators: Role of A1 Agonists and A2A Antagonists. Cells
. 2020 Jul 21;9(7):1739. IF ; rat.
[IF=2.34] Cao, Zhi-Ping, et al. "Effects of cordycepin on spontaneous alternation behavior and adenosine receptors expression in hippocampus." Physiology & Behavior (2017). IHC-P ; Mouse.
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| 研究領域 | 心血管 細胞生物 信號轉導 細胞凋亡 轉錄調節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 45kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human A2AR: 165-300/412 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein, an adenosine receptor of A2A subtype, uses adenosine as the preferred endogenous agonist and preferentially interacts with the G(s) and G(olf) family of G proteins to increase intracellular cAMP levels. It plays an important role in many biological functions, such as cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. It has been implicated in pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders. Alternative splicing results in multiple transcript variants. A read-through transcript composed of the upstream SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding. [provided by RefSeq, Jun 2013] Function: Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Subunit: Interacts (via cytoplasmic C-terminal domain) with USP4; the interaction is direct. May interact with DRD4. Subcellular Location: Cell membrane; Multi-pass membrane protein. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P29274 Gene ID: 135 Database links: Entrez Gene: 135 Human Entrez Gene: 11540 Mouse Omim: 102776 Human SwissProt: P29274 Human SwissProt: Q60613 Mouse Unigene: 197029 Human Unigene: 333734 Mouse Unigene: 11180 Rat 腺苷A2A受體參與神經遞質運輸、吞噬、細胞凋亡、炎癥應答、細胞防御應答、信號轉導。 腺苷是腺嘌呤核苷酸代謝的中間產物。是一種保護正常組織免于炎癥損傷的關鍵的、多余的負調節(jié)免疫細胞。由cAMP 生物合成。 |
| 產品圖片 |
Sample: placenta (Mouse) Lysate at 40 ug
Primary: Anti-Adenosine Receptor A2a (bs-1456R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 45kD
Sample:
MOlt4(Human) Cell Lysate at 30 ug
Raji Cell Lysate at 30 ug
Primary: Anti-Adenosine Receptor A2a (bs-1456R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 50 kD
Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Thymus (Mouse) Lysate at 40 ug
Lane 3: Cerebrum (Rat) Lysate at 40 ug
Lane 4: Thymus (Rat) Lysate at 40 ug
Lane 5: Lymph node (Rat) Lysate at 40 ug
Lane 6: SH-SY5Y (Human) cell Lysate at 30 ug
Lane 7: Molt-4(Human) cell Lysate at 30 ug
Lane 8: U87MG (Human) cell Lysate at 30 ug
Lane 9: Huvec (Human) cell Lysate at 30 ug
Primary: Anti-Adenosine Receptor A2a (bs-1456R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 47 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
