| 產(chǎn)品編號 | bs-1704R |
| 英文名稱 | Rabbit Anti-PAI1 antibody |
| 中文名稱 | 纖溶酶原激活物抑制因子抗體 |
| 別 名 | Clade E; Endothelial plasminogen activator inhibitor; Nexin; PAI 1; PAI; PAI-1; PAI1_HUMAN; PLANH1; Plasminogen activator inhibitor 1; Plasminogen activator inhibitor type 1; Serine (or cysteine) proteinase inhibitor; Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1; Serpin E1; Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1; Serpin peptidase inhibitor clade E; Serpine 1; Serpine1. |
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Specific References (1) | bs-1704R has been referenced in 1 publications.
[IF=0.826] Keiko M et al. Cultured Neonatal Rat Cardiomyocytes Continue Beating Through Upregulation of CTGF Gene ExpressionJ Nippon Med Sch.2020;87(5):268-276. WB ; Rat.
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| 研究領(lǐng)域 | 腫瘤 心血管 細胞生物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 43kDa |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PAI-1: 301-402/402 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
PAI1 (plasminogen activator inhibitor 1) is originally cloned from human endothelial cell (Pannekoek 1986, Ginsburg 1986) and rat hepatoma cell 3 cDNA libraries. As a member of the serpin family of serine protease inhibitors, PAI1 inhibits both tissue type plasminogen activator (tPA) and urokinase type plasminogen activator (uPA). High PAI1 levels are associated with an increased risk of thromboembolic disease while PAI1 deficiency may represent an inherited autosomal recessive bleeding disorder. Function: Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis. Subunit: Forms protease inhibiting heterodimer with TMPRSS7. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Subcellular Location: Secreted Tissue Specificity: Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells Post-translational modifications: Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-|-Met-370 bond. DISEASE: Plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]: A hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen. Note=The disease is caused by mutations affecting the gene represented in this entry. Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions. Similarity: Belongs to the serpin family SWISS: P05121 Gene ID: 5054 Database links: Entrez Gene: 5054 Human Entrez Gene: 18787 Mouse Omim: 173360 Human SwissProt: P05121 Human SwissProt: P22777 Mouse Unigene: 414795 Human Unigene: 713079 Human Unigene: 250422 Mouse Unigene: 29367 Rat PAI-1是一種單鏈糖蛋白，其生理作用很多： （1）與組織型纖溶酶原激活物（tissue plasminogen activator，t-PA）形成1：1的復(fù)合物而使t-PA失活; （2）保護基底膜不被血漿來源的酶所降解; （3）保護細胞間的接觸面而維持組織結(jié)構(gòu)的完整性; （4）在結(jié)締組織演變、凝血、纖溶、補體激活和炎癥反應(yīng)等過程中具有抑制蛋白降解作用; （5）在細胞周期中，PAI-1轉(zhuǎn)錄水平的變化及其在細胞內(nèi)的積聚，對細胞形態(tài)的維持、細胞與其間質(zhì)的粘附、細胞增殖、信號轉(zhuǎn)導(dǎo)及基因表達等都有重要意義; |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Lung tissue lysates
Lane 2: Rat Lung tissue lysates
Primary: Anti-PAI1 (bs-1704R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 46 kDa
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PAI1) polyclonal Antibody, Unconjugated (bs-1704R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PAI1) polyclonal Antibody, Unconjugated (bs-1704R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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