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Rabbit Anti-Met Enkephalin  antibody (bs-1759R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號 bs-1759R
英文名稱 Rabbit Anti-Met Enkephalin  antibody
中文名稱 甲硫氨酸腦啡肽抗體
別    名 Met-enkephalin; [Met]enkephalin; Enkephalin; M-ENK; Methionine Enkephalin; OGF; Opioid growth factor; PENK; PPA; Proenkephalin A; Proenkephalin; M enk; PE; PENK-A; PENK_HUMAN.  
Specific References  (1)     |     bs-1759R has been referenced in 1 publications.
[IF=38.138] Brestoff, Jonathan R., et al. "Group 2 innate lymphoid cells promote beiging of white adipose tissue and limit obesity." Nature (2014).  FCM ;  Human.  
研究領(lǐng)域 神經(jīng)生物學(xué)  生長因子和激素  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human (predicted: Mouse,Rat)
產(chǎn)品應(yīng)用 Flow-Cyt=2ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 .5kDa
細(xì)胞定位 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated Met Enkephalin peptide (Tyr-Gly-Gly-Phe-Met) 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Enkephalin and enkephalin-like peptides have been detected in many animal species. In mammals these opioid pentapeptides are cleaved from a common precursor molecule which is expressed in a number of cell types, including neurons and endocrine cells associated with the sympathetic nervous system where they are often co-stored with catecholamines. They are known to be endogenous ligands for Sopioid receptors and are known to mediate analgesia at the spinal level.
Met-enkephalin (tyr-gly-gly-phe-met) and leu-enkephalin (tyr-gly-gly-phe-leu) are pentapeptides which compete with and mimic the effects of opiate drugs. Although interest in enkephalins stems largely from their possible role in the brain, the richest source of these peptides is the adrenal gland. The amino acid sequence shows that the precursor is 267 amino acids long and contains 6 interspersed Met-enkephalin sequences and 1 Leu-enkephalin sequence. The precursor does not contain the sequences of dynorphin, alpha-neo-endorphin or beta-endorphin. (Because of structural similarities it had been postulated that beta-endorphin is precursor of Met-enkephalin, and that dynorphin or alpha-neo-endorphin is precursor of Leu-enkephalin.)

Function:
Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.

Subcellular Location:
Secreted.

Post-translational modifications:
The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.

Similarity:
Belongs to the opioid neuropeptide precursor family.

SWISS:
P01210

Gene ID:
5179

Database links:

Entrez Gene: 5179 Human

Entrez Gene: 18619 Mouse

Entrez Gene: 29237 Rat

Omim: 131330 Human

SwissProt: P01210 Human

SwissProt: P22005 Mouse

SwissProt: P04094 Rat

Unigene: 339831 Human

Unigene: 475097 Mouse

Unigene: 10015 Rat



產(chǎn)品圖片
Blank control(black line):SH-SY5Y. Primary Antibody (green line): Rabbit Anti-Met Enkephalin antibody (bs-1759R) Dilution:2ug/Test; Secondary Antibody(white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control(orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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