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Rabbit Anti-STEAP1  antibody (bs-1901R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號 bs-1901R
英文名稱 Rabbit Anti-STEAP1  antibody
中文名稱 前列腺跨膜上皮1抗原抗體
別    名 Six transmbrane epithelial antigen of the prostate; MGC19484; PRSS 24; PRSS24; Six transmembrane epithelial antigen of the prostate; STEAP 1; STEAP1; STEA1_HUMAN.  
研究領(lǐng)域 腫瘤  免疫學(xué)  上皮細(xì)胞  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human (predicted: Mouse,Rat,Cow,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 40kDa
細(xì)胞定位 細(xì)胞漿 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human STEAP1: 251-339/339 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene is predominantly expressed in prostate tissue, and is found to be upregulated in multiple cancer cell lines. The gene product is predicted to be a six transmembrane protein, and was shown to be a cell surface antigen significantly expressed at cell cell junctions.
Prostate cancer is the most frequently diagnosed cancer and is the second leading cause of cancer-related deaths in American men. Although the early serum detection of prostate-specific antigen (PSA) plays important roles in early diagnosis, it may not distinguish prostate cancer from benign diseases. Recently, a novel protein, which contains 339 amino acids, named STEAP (six transmembrane epithelial antigen of the prostate), was identified in advanced prostate cancer. STEAP is unique among the currently known prostate cancer markers because of its putative secondary structure, from which one may predict that it functions as a potential channel protein. STEAP is strongly expressed in advanced prostate cancer and some extent expression in other cancers, such as colon and ovarian cancer cell lines.

Function:
Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor.

Subcellular Location:
Endosome membrane; Multi-pass membrane protein.

Tissue Specificity:
Ubiquitously expressed. Highly expressed in prostate tumors.

Similarity:
Belongs to the STEAP family.
Contains 1 ferric oxidoreductase domain.

SWISS:
Q9UHE8

Gene ID:
26872

Database links:

Entrez Gene: 26872 Human

Entrez Gene: 297738 Rat

Omim: 604415 Human

SwissProt: Q9UHE8 Human

Unigene: 61635 Human

Unigene: 51773 Rat



產(chǎn)品圖片
Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-STEAP1 (bs-1901R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 40 kD
Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-STEAP1 Polyclonal Antibody, Unconjugated(bs-1901R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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