| 產(chǎn)品編號 | bs-2407R |
| 英文名稱 | Rabbit Anti-SV2A antibody |
| 中文名稱 | 突觸泡蛋白2A抗體 |
| 別 名 | Synaptic vesicle protein 2A; Synaptic vesicle glycoprotein 2A; Synaptic vesicle protein 2A; Synaptic vesicle protein 2; Sv2; Kiaa0736; SV2A_HUMAN. |
| 研究領(lǐng)域 | 神經(jīng)生物學 細胞膜受體 糖蛋白 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應用 | Flow-Cyt=1ug/test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 83kDa |
| 細胞定位 | 細胞漿 細胞膜 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SV2A: 451-550/743 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
SV2s (Synaptic Vesicle protein 2) are integral membrane glycoproteins present in all synaptic vesicles. They have 12 transmembrane domains predicted by sequence analysis. There are three characterized isoforms, SV2A, SV2B and SV2C. SV2A is expressed ubiquitously throughout the brain. SV2B has a more restricted distribution with varying degrees of coexpression with SV2A. SV2C is more closely related to SV2A but shows a very restricted expression pattern. The highest expression levels were observed in phylogenetically old brain areas like pallidum, the midbrain and the olfactory bulb. SV2A is probably involved in the maintenance of a pool of synaptic vesicles competent for calcium-stimulated exocytosis. SV2A is a highly glycosylated synaptic vesicle protein with homology to transmembrane transporters. Function: Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles (By similarity). Subunit: Interacts with SYT1/synaptotagmin-1 in a calcium-dependent manner. Binds the adapter protein complex AP-2. Subcellular Location: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein. Post-translational modifications: Phosphorylation by CK1 of the N-terminal cytoplasmic domain regulates interaction with SYT1. N-glycosylated. Similarity: Belongs to the major facilitator superfamily. SWISS: Q7L0J3 Gene ID: 9900 Database links: Entrez Gene: 9900 Human Entrez Gene: 64051 Mouse Omim: 185860 Human SwissProt: Q7L0J3 Human SwissProt: Q9JIS5 Mouse Unigene: 516153 Human Unigene: 200365 Mouse Unigene: 11264 Rat 突觸泡蛋白2A(SV2A)是一類跨膜糖蛋白,定位于脊椎動物神經(jīng)元及內(nèi)分泌細胞,與神經(jīng)遞質(zhì)的釋放、內(nèi)分泌泡胞吐作用、突觸泡穩(wěn)態(tài)的維持作用。 |
| 產(chǎn)品圖片 |
Blank control:Molt-4.
Primary Antibody (green line): Rabbit Anti-SV2A antibody (bs-2407)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Black line : Positive blank control (Molt4); Negative blank control (A549）
Green line : Primary Antibody (Rabbit Anti- SV2A antibody (bs-2407R) )
Orange line：Isotype Control Antibody (Rabbit IgG) .
Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF488)/(Goat anti-rabbit IgG-AF647)
Molt4（Positive）and A549（Negative control）cells (black) were fixed with 4% PFA for 10min at room temperature, permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SV2A Antibody(bs-2407R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
