產(chǎn)品編號 | bs-2676R |
英文名稱 | Rabbit Anti-TSHB antibody |
中文名稱 | 促甲狀腺素β亞單位抗體 |
別 名 | TSHB_HUMAN; Thyrotropin subunit beta; Thyroid-stimulating hormone subunit beta (TSH-B; TSH-beta); Thyrotropin beta chain; Thyrotropin alfa; TSH-β; |
Specific References (1) | bs-2676R has been referenced in 1 publications.
[IF=5.02] Ikeda et al. Expression of Kisspeptin in Gonadotrope Precursors in the Mouse Pituitary during Embryonic and Postnatal Development and in Adulthood. (2016) Neuroendocrinology. IHC ; Mouse.
|
|
研究領(lǐng)域 | 生長因子和激素 內(nèi)分泌病 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Horse) |
產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 12kDa |
細胞定位 | 分泌型蛋白 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human TSHB: 51-138/138 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. Thyroid stimulating hormone functions in the control of thyroid structure and metabolism. The protein encoded by this gene is the beta subunit of thyroid stimulating hormone. Mutations in this gene are associated with congenital central and secondary hypothyroidism and Hashimoto's thyroiditis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013] Function: Indispensable for the control of thyroid structure and metabolism. Subunit: Heterodimer of a common alpha chain and a unique beta chain which confers biological specificity to thyrotropin, lutropin, follitropin and gonadotropin. Subcellular Location: Secreted. Similarity: Belongs to the glycoprotein hormones subunit beta family. SWISS: P01222 Gene ID: 7252 Database links:
Entrez Gene: 7252 Human SwissProt: P01222 Human
|
產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse pituitary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TSH) Polyclonal Antibody, Unconjugated (bs-2676R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
1、抗體溶解方法 | |
2、抗體修復(fù)方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關(guān)于肽鏈的設(shè)計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |