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Rabbit Anti-Phospho-ATP1A1 (Ser16)  antibody (bs-3035R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號(hào) bs-3035R
英文名稱 Rabbit Anti-Phospho-ATP1A1 (Ser16)  antibody
中文名稱 磷酸化鈉鉀ATP酶蛋白a1抗體
別    名 alpha 1 Sodium Potassium ATPase (phospho S16); alpha 1 Sodium Potassium ATPase; A1A1; AT1A1; AT1A1_HUMAN; Atpa-1; ATPase Na+/K+ transporting alpha 1 polypeptide; ATPase Na+/K+ transporting subunit alpha 1; BC010319; EC 3.6.3.9; MGC3285; MGC38419; MGC51750; Na K ATPase alpha A catalytic polypeptide; Na K ATPase catalytic subunit alpha A protein; Na(+)/K(+) ATPase 1; Na(+)/K(+) ATPase alpha-1 subunit; Na+, K+ ATPase alpha subunit; Na+/K+ ATPase alpha 1 subunit; Na+/K+ ATPase 1; Na,K ATPase alpha 1 subunit; Nkaa1b; Sodium potassium ATPase alpha 1 polypeptide; Sodium pump 1; Sodium pump subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-1; Sodium/potassium-transporting ATPase subunit alpha-1.  
產(chǎn)品類型 磷酸化抗體 
研究領(lǐng)域 通道蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human (predicted: Mouse,Rat,Pig,Sheep,Cow,Dog,GuineaPig)
產(chǎn)品應(yīng)用 WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 110kDa
細(xì)胞定位 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human ATP1A1 around the phosphorylation site of Ser16: AV(p-S)E 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+-ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May2009].

Function:
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

Subunit:
Interacts with SIK1. Composed of three subunits: alpha (catalytic), beta and gamma. Binds the HLA class II histocompatibility antigen, DR1.

Subcellular Location:
Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Post-translational modifications:
Phosphorylation on Tyr-10 modulates pumping activity. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity.

Similarity:
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.

SWISS:
P05023

Gene ID:
476

Database links:

Entrez Gene: 476 Human

Entrez Gene: 11928 Mouse

Entrez Gene: 24211 Rat

Omim: 182310 Human

SwissProt: P05023 Human

SwissProt: Q8VDN2 Mouse

SwissProt: P06685 Rat

Unigene: 371889 Human

Unigene: 280103 Mouse

Unigene: 217534 Rat

Unigene: 2992 Rat



通道蛋白(Channel Protein)
鈉鉀ATP酶是位于細(xì)胞膜上的一種糖蛋白,與ATP 的分解和細(xì)胞內(nèi)外鈉、鉀離子的轉(zhuǎn)運(yùn)密切相關(guān),哺乳動(dòng)物各種組織細(xì)胞的鈉鉀ATP 酶的免疫學(xué)特性基本相同。
產(chǎn)品圖片
Sample: Lane 1: Human Jurkat-TPA cell Lysates Lane 2: Human 293T-TPA cell Lysates Primary: Anti-Phospho-ATP1A1 (Ser16)(bs-3035R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 110kDa Observed band size: 130kDa
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