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Rabbit Anti-Afadin  antibody (bs-2754R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號(hào) bs-2754R
英文名稱 Rabbit Anti-Afadin  antibody
中文名稱 絲狀肌動(dòng)蛋白結(jié)合蛋白抗體
別    名 AF 6; AF6; AFAD_HUMAN; Afadin; ALL1-fused gene from chromosome 6 protein; FLJ34371; MLLT 4; MLLT4; Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog Drosophila) translocated to 4; Myeloid/lymphoid or mixed lineage leukemia translocated to 4; Protein AF 6; Protein Af-6; Protein AF6; RP3 431P23.3; 5033403D15Rik; Gm314; I-afadin; Mllt4; S-afadin.  
研究領(lǐng)域 神經(jīng)生物學(xué)  信號(hào)轉(zhuǎn)導(dǎo)  細(xì)胞粘附分子  細(xì)胞骨架  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Mouse,Rat (predicted: Human,Pig,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 207kDa
細(xì)胞定位 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Afadin: 341-440/1824 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Afadin is a cell-cell adherens junction F-actin binding multidomain protein. Two splice variants of afadin have been described: l-afadin, which is ubiquitously expressed, and the smaller s-afadin, which is abundantly expressed in neural tissue. l-Afadin contains one PDZ domain near its middle followed by three proline-rich and one F-actin-binding region at the carboxyl-terminal. l/s-Afadin is localized to cell-cell junctions and binds several cytoplasmic proteins such as the small GTPase Ras, the tight junction protein ZO-1, and the vinculin binding protein ponsin/SH3P12.

Function:
Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton.

Subunit:
Homodimer. Interacts with F-actin, nectin and PVRL3/nectin-3. Essential for the association of nectin and E-cadherin. Isoform 1/s-afadin does not interact with F-actin. Interacts with ZO-1 and occludin, but probably in an indirect manner. Interacts with RIT1 and RIT2 (By similarity). Interacts with NRXN1 and BCR.

Subcellular Location:
Cell junction, adherens junction. Note=Not found at cell-matrix AJs.

DISEASE:
Note=A chromosomal aberration involving MLLT4 is associated with acute leukemias. Translocation t(6;11)(q27;q23) with MLL/HRX. The result is a rogue activator protein.

Similarity:
Contains 1 dilute domain.
Contains 1 FHA domain.
Contains 1 PDZ (DHR) domain.
Contains 2 Ras-associating domains

SWISS:
P55196

Gene ID:
4301

Database links:

Entrez Gene: 4301 Human

Entrez Gene: 17356 Mouse

Entrez Gene: 26955 Rat

Omim: 159559 Human

SwissProt: P55196 Human

SwissProt: Q9QZQ1 Mouse

SwissProt: O35889 Rat

Unigene: 614974 Human

Unigene: 644024 Human

Unigene: 708726 Human

Unigene: 728849 Human

Unigene: 59167 Mouse

Unigene: 58 Rat



產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Afadin) Polyclonal Antibody, Unconjugated (bs-2754R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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