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Rabbit Anti-Phospho-cdc25C (Ser216)  antibody (bs-3096R)  
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產品編號 bs-3096R
英文名稱 Rabbit Anti-Phospho-cdc25C (Ser216)  antibody
中文名稱 磷酸化細胞分裂周期蛋白25C抗體
別    名 cdc25C(Phospho-Ser216); cdc25C(Phospho Ser216); cdc25C(Phospho S216); CDC 25; Cdc 25C; CDC25; CDC25C; Cell division cycle 25 homolog C; Cell Division Cycle 25C; Cell Division Cycle 25C; Cell division cycle 25C protein; Dual specificity phosphatase Cdc25C; M phase inducer phosphatase 3; M-phase inducer phosphatase 3; Mitosis inducer CDC25; MPIP3; MPIP3_HUMAN; Phosphotyrosine phosphatase.  
Specific References  (1)     |     bs-3096R has been referenced in 1 publications.
[IF=2.976] Chen X et al. Reactive oxygen species induced by icaritin promote DNA strand breaks and apoptosis in human cervical cancer cells.(2019)Oncol Rep. Feb;41(2):765-778.  WB ;  
產品類型 磷酸化抗體 
研究領域 細胞生物  染色質和核信號  信號轉導  細胞周期蛋白  激酶和磷酸酶  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 (predicted: Human,Mouse,Rat)
產品應用 ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 53kDa
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human cdc25C around the phosphorylation site of Ser216: P(p-S)MP 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. [provided by RefSeq, Jul 2008]

Function:
Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.

Subunit:
Interacts with HIV-1 Vpr, thereby inactivating CDC25C phosphatase activity. Interacts with MAPK14 and 14-3-3 proteins.

Subcellular Location:
Nucleus.

Post-translational modifications:
Phosphorylated by CHEK1 and MAPK14 at Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-191 promotes nuclear translocation. Ser-198 is a minor phosphorylation site. Was initially reported to be phosphorylated by PLK3 at Ser-216 (PubMed:10557092). However, such phosphorylation by PLK3 was not confirmed by other groups. Phosphorylation at Thr-48, Thr-67, Ser-122, Thr-130, Ser-168 and Ser-214 occurs at G2 and G2-M transition and is probably catalyzed by CDK1. Ser-168 phosphorylation levels are lower than those at the other 5 CDK1 sites. Phosphorylation by CDK1 leads to increased activity.

Similarity:
Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain.

SWISS:
P30307

Gene ID:
995

Database links:

Entrez Gene: 995 Human

Omim: 157680 Human

SwissProt: P30307 Human

Unigene: 656 Human



CDC25家族的組成哺乳動物中CDC25家族一共包括3種同源異構體(CDC25A、CDC25B、CDC25C),約有50%的序列同源,是一組在細胞周期調控中發(fā)揮巨大作用的蘇/酪氨酸雙功能酶 。不同的CDC25家族蛋白在細胞周期中的作用時相亦有差異,CDC25A和CDC25C分別在S期和M期發(fā)揮主要作用。
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