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Rabbit Anti-DARPP32  antibody (bs-2762R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號 bs-2762R
英文名稱 Rabbit Anti-DARPP32  antibody
中文名稱 多巴胺、cAMP調節(jié)磷蛋白抗體
別    名 DARPP 32; DARPP-32; Dopamine and cAMP regulated neuronal phosphoprotein; Dopamine and cAMP regulated phosphoprotein DARPP 32; Dopamine and cAMP regulated phosphoprotein DARPP32; FLJ20940; IPPD; Neuronal phosphoprotein DARPP 32; PPP1R1B; Protein phosphatase 1 regulatory (inhibitor) subunit 1B; Protein phosphatase 1 regulatory subunit 1B.  
研究領域 神經(jīng)生物學  信號轉導  通道蛋白  細胞膜受體  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Rat,GuineaPig (predicted: Human,Mouse,Rabbit,Pig,Cow,Dog,Horse)
產(chǎn)品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 23kDa
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human DARPP32: 51-150/204 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011].

Function:
Inhibitor of protein-phosphatase 1.

Subcellular Location:
Cytoplasm.

Post-translational modifications:
Phosphorylation of Thr-34 is required for activity.

Similarity:
Belongs to the protein phosphatase inhibitor 1 family.

SWISS:
Q9UD71

Gene ID:
84152

Database links:

Entrez Gene: 84152 Human

Entrez Gene: 19049 Mouse

Entrez Gene: 360616 Rat

Omim: 604399 Human

SwissProt: Q9UD71 Human

SwissProt: Q60829 Mouse

SwissProt: Q6J4I0 Rat

Unigene: 286192 Human

Unigene: 45372 Mouse

Unigene: 70366 Rat



????DARPP-32通過自身不同位點的磷酸化對蛋白質磷酸化和去磷酸化過程發(fā)揮著雙向調控的作用.DARRP-32在腦內存在于接受多巴胺能投射的神經(jīng)元中,其磷酸化過程受多種神經(jīng)遞質的調控,在信號轉導途徑中處于中心位置,整合多種胞內信號轉導,調節(jié)神經(jīng)元的電學和化學性質以及動物的生理行為反應,參與多種生理功能和病理過程,包括藥物成癮、抑郁癥、精神分裂癥等.此外,DARPP-32在非神經(jīng)系統(tǒng)的功能也逐漸被人們所認識.
????多巴胺和cAMP調節(jié)的磷蛋白(dopamine and adenosine 3'5'-monophosphate-regulated phospho-protein,Mr 32 kD,DARPP-32)是一個具有雙重功能的獨特蛋白,它既是磷酸酶(如PP-1)的抑制劑,也是蛋白激酶(如PKA)的抑制劑。
產(chǎn)品圖片
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-DARPP32 Polyclonal Antibody, Unconjugated(bs-2762R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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