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Rabbit Anti-CYP2R1  antibody (bs-3900R)  
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產(chǎn)品編號 bs-3900R
英文名稱 Rabbit Anti-CYP2R1  antibody
中文名稱 細(xì)胞色素P450 2R1抗體
別    名 Cytochrome P450 2R1; Cytochrome P450 family 2 R1; Cytochrome P450 family 2 subfamily R polypeptide 1; EC 1.14.13.15; MGC4663; Vitamin D 25 hydroxylase.  
Specific References  (1)     |     bs-3900R has been referenced in 1 publications.
[IF=4.285] Stenhouse Claire. et al. Effects of progesterone and interferon tau on ovine endometrial phosphate, calcium, and vitamin D signaling. Biol Reprod. 2022 Feb;:  IHC ;  Sheep(Ewe).  
研究領(lǐng)域 腫瘤  心血管  細(xì)胞生物  免疫學(xué)  信號轉(zhuǎn)導(dǎo)  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse (predicted: Rat,Rabbit,Pig,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,Flow-Cyt=2ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 55kDa
細(xì)胞定位 細(xì)胞漿 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CYP2R1: 251-350/501 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 CYP2R1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are mono-oxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme is a microsomal vitamin D hydroxylase that converts vitamin D into the active ligand for the vitamin D receptor. Defects in CYP2R1 are a cause of 25-hydroxyvitamin D(3) deficiency, also known as pseudovitamin D(3) deficiency rickets due to 25-hydroxylase deficiency. First described in patients who had rickets at a young age despite a history of adequate vitamin D intake. The patients sera had low calcium concentrations, low phosphate concentrations, elevated alkaline phosphatase activity and low levels of 25-hydroxyvitamin D.

Subunit:
Homodimer.

Subcellular Location:
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue Specificity:
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Similarity:
Belongs to the cytochrome P450 family.

SWISS:
Q6VVX0

Gene ID:
120227

Database links:

Entrez Gene: 120227 Human

Entrez Gene: 244209 Mouse

Omim: 608713 Human

SwissProt: Q6VVX0 Human

SwissProt: Q6VVW9 Mouse

Unigene: 371427 Human

Unigene: 108037 Mouse



產(chǎn)品圖片
Sample: A431(Human) Cell Lysate at 30 ug Primary: Anti-CYP2R1 (bs-3900R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD
Sample: A431(Human) Cell Lysate at 30 ug Primary: Anti- CYP2R1 (bs-3900R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 53 kD
Sample: Liver(Human) Cell Lysate at 40 ug Primary: Anti-CYP2R1 (bs-3900R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD
Blank control:Jurkat. Primary Antibody (green line): Rabbit Anti-CYP2R1 antibody (bs-3900R) Dilution: 2ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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