| 產(chǎn)品編號 | bsF-P0002 |
| 英文名稱 | KLH / FITC |
| 中文名稱 | FITC標記血藍蛋白 |
| 別 名 | Keyhole limpet; keyhole limpet hemocyanin; Hemocyanin from Megathura crenulata (keyhole limpet); Hemocyanin from Limulus polyphemus hemolymph; hemocyanin; haemocyanin; KLH / FITC 血藍素; 鑰孔血藍蛋白; |
| 性 狀 | Liquid |
| 濃 度 | >1.0 mg/ml |
| 物 種 | N/A |
| 序 列 | Purified native protein |
| 內(nèi)毒素 | Not analyzed |
| 活性 | Not tested |
| 緩 沖 液 | 10 mM TBS (pH=7.4) with 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
Keyhole limpet hemocyanin is an extremely large, heterogeneous glycosylated protein consisting of subunits with a molecular weight of 350,000 and 390,000 in aggregates with molecular weights of 4,500,000-13,000,000. Each domain of a KLH subunit contains two copper atoms that together bind a single oxygen molecule (O2). When oxygen is bound to hemocyanin, the molecule takes on a distinctive transparent, opalescent blue color. The KLH protein is potently immunogenic yet safe in humans and is therefore highly prized as a vaccine carrier protein. The large and highly glycosylated KLH protein cannot be reproduced synthetically. It is available only as a purified biological product from the Keyhole Limpet Megathura crenulata. Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein in the production of antibodies for research, biotechnology and therapeutic applications. Haptens are substances with a low molecular weight such as peptides, small proteins and drug molecules that are generally not immunogenic and require the aid of a carrier protein to stimulate a response from the immune system in the form of antibody production. KLH is the most widely employed carrier proteins for this purpose. KLH is an effective carrier protein for several reasons. Its large size and numerous epitopes generate a substantial immune response, and abundance of lysine residues for coupling haptens, allows a high hapten:carrier protein ratio increasing the likelihood of generating hapten-specific antibodies. In addition, because KLH is derived from the limpet, a gastropod, it is phylogenetically distant from mammalian proteins, thus reducing false positives in immunologically based research techniques in mammalian model organisms. KLH may also be a challenging molecule to work with because of its propensity to aggregate and precipitate. Aggregates remain immunogenic, but limit the ability to conjugate haptens and are difficult to manipulate in the laboratory. A high quality KLH preparation with the clear opalescent blue color is the best indicator of KLH solubility. ?????????????????????????綠色熒光素（FITC）標記標記血藍蛋白 ?????????????????????????????產(chǎn) 品 說 明 ????熒光素標記抗體（FA）是目前廣泛用于免疫病理、細胞化學、流氏細胞學、病理學及自身抗體的臨床免疫中的特異、靈敏、定性和定位相結合的免疫化學試劑。 ????我公司的熒光素抗體為本公司制備的抗血清，再經(jīng)過親和層析純化或特異性免疫親和層析純化的方法純化為抗體的IgG成分，并采用直接標記法，將異硫氰酸熒光素（FITC美國sigma熒光素產(chǎn)品）共價交聯(lián)到抗體血管緊張素轉換酶（IgG）分子的賴氨酸分子上，經(jīng)過標記偶聯(lián)、低溫透析、HPLC分離純化、分子篩過濾、濃縮管濃縮、蛋白定量、標記后濃度鑒定、檢測、分裝、真空冷凍抽干而制備完成。 ????應用范圍： ????1.免疫組織及免疫病理中抗原、抗體檢查，如：各腫瘤抗原檢查，細胞凋亡檢測。 ????2.用于細胞因子等檢查的熒光流式細胞儀測定。 ????3.用于臨床自身免疫病的多種自身抗體熒光檢查，如：抗核抗體，抗心肌酶抗體. ????4.白細胞分型抗原的免疫熒光染色。 ????5.用于細菌、病毒、梅毒螺旋體、鉤端螺旋體等微生物的抗原及抗體檢測。 ????主要性能： ????0.5ml或根據(jù)試驗需求的各種包裝，并提供濃縮液或凍干粉，含有0.01%NaN3防腐劑。 ????血藍蛋白含量在1mg/1ml左右.FITC為Sigma異構體I,約在70-80ug/ml,495nm為最大吸收峰，激發(fā)波長 525 nm . ????工作濃度： ????用0.01mol/l pH7.4 PBS 作1：20–1：50稀釋使用。 ????保存：-20°C可保存一年，避免反復凍融。 ????我公司可根據(jù)您科研的需求提供各種標記服務 ，標記各種符合標記條件的蛋白及抗體，質(zhì)量保證，用時短，價格合理。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
