| 產(chǎn)品編號(hào) | bs-2382R |
| 英文名稱 | Rabbit Anti-ST2 antibody |
| 中文名稱 | 白細(xì)胞介素1受體相關(guān)蛋白抗體 |
| 別 名 | DER-4; DER4; FIT 1; Growth stimulation expressed gene 2; homolog of mouse growth stimulation-expressed; Il1rl1; IL33R; ILRL1_HUMAN; Interleukin 1 receptor like 1; interleukin 1 receptor related protein; Interleukin-1 receptor-like 1; Ly84; Lymphocyte antigen 84; Protein ST2; ST2; ST2L; ST2V; Ste2; Suppressor of tumorigenicity 2; T1; T1 protein. 生長(zhǎng)刺激表達(dá)基因2 |
 |
Specific References (2) | bs-2382R has been referenced in 2 publications.
[IF=6.22] Li et al. IL-33 facilitates proliferation of colorectal cancer dependent on?COX2/PGE2. (2018) J.Exp.Clin.Cancer.Res. 37:196 FCM ; Mouse.
[IF=3.53] Yang S-H, Lin J-C, Wu S-Y, Huang K-L, Jung F, et al. (2015) Membrane Translocation of IL-33 Receptor in Ventilator Induced Lung Injury. PLoS ONE 10(3): e0121391. WB ; Rat.
|
| 研究領(lǐng)域 | 心血管 細(xì)胞生物 免疫學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse,Pig,Cow,Dog) |
| 產(chǎn)品應(yīng)用 | Flow-Cyt=2μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 61kDa |
| 細(xì)胞定位 | 細(xì)胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Interleukin 1 receptor like 1: 11-110/556 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
ST2 is a member of a superfamily containing the Interleukin 1 (IL1) receptor and the Toll-like receptors (TLRs). The TLRs are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. ST2 was originally identified as a protein whose production was stimulated by various proliferation-inducing agents such as PDGF and FGF. More recently, it has been shown to negatively regulate IL1 receptor and Toll-like receptor 4 (TLR4) signaling and to maintain endotoxin tolerance. It has been suggested that the inhibition of TLR4 signaling occurs through the association and sequestering of TLR adaptor molecules such as MyD88 and TIRAP. Function: Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function. Subunit: Interacts with MYD88, IRAK1, IRAK4, and TRAF6. Subcellular Location: Isoform C: Cell membrane. Isoform B: Secreted. Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Highly expressed in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine. Isoform A is prevalently expressed in the lung, testis, placenta, stomach and colon. Isoform B is more abundant in the brain, kidney and the liver. Isoform C is not detected in brain, heart, liver, kidney and skeletal muscle. Similarity: Belongs to the interleukin-1 receptor family. Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 TIR domain. SWISS: Q01638 Gene ID: 9173 Database links: Entrez Gene: 9173 Human Entrez Gene: 17082 Mouse Omim: 601203 Human SwissProt: Q01638 Human SwissProt: P14719 Mouse Unigene: 66 Human Unigene: 289824 Mouse |
| 產(chǎn)品圖片 |
Blank control: HUVEC.
Primary Antibody (green line): Rabbit Anti-ST2 antibody (bs-2382R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Black line : Positive blank control (HUVEC); Negative blank control (MCF7）
Green line : Primary Antibody (Rabbit Anti-ST2 antibody (bs-2382R) )
Orange line：Isotype Control Antibody (Rabbit IgG) .
Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF647)
HUVEC（Positive）and MCF7（Negative control）cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ST2 Antibody(bs-2382R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
|
