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Rabbit Anti-FAT  antibody (bs-4278R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號(hào) bs-4278R
英文名稱 Rabbit Anti-FAT  antibody
中文名稱 鈣粘蛋白家族成員7抗體
別    名 Cadherin family member 7; cadherin family member 7 precursor; cadherin ME5; Cadherin related tumor suppressor; Cadherin related tumor suppressor homolog; cadherin related tumor suppressor homolog precursor; Cadherin related tumor suppressor homolog precursor (FAT protein homolog); CDHF 7; CDHF7; FAT 1; FAT tumor suppressor 1; FAT tumor suppressor homolog 1; FAT1; hFat 1; hFAT; hFat1; homolog of Drosophila FAT protein; homolog of Drosophila FAT protein precursor; homolog of Drosophila tumor suppressor FAT precursor; ME 5; ME5; Protein fat homolog.  
研究領(lǐng)域 腫瘤  細(xì)胞生物  免疫學(xué)  細(xì)胞周期蛋白  細(xì)胞粘附分子  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Rat (predicted: Human,Mouse,Pig,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 506kDa
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human FAT/CDHF7: 1401-1500/4588 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene is an ortholog of the Drosophila fat gene, which encodes a tumor suppressor essential for controlling cell proliferation during Drosophila development. The gene product is a member of the cadherin superfamily, a group of integral membrane proteins characterized by the presence of cadherin-type repeats. In addition to containing 34 tandem cadherin-type repeats, the gene product has five epidermal growth factor (EGF)-like repeats and one laminin A-G domain. This gene is expressed at high levels in a number of fetal epithelia. Its product probably functions as an adhesion molecule and/or signaling receptor, and is likely to be important in developmental processes and cell communication. Transcript variants derived from alternative splicing and/or alternative promoter usage exist, but they have not been fully described. [provided by RefSeq].

Function:
Plays an essential role for cellular polarization, directed cell migration and modulating cell-cell contact.

Subunit:
Interacts (via the C-terminus 4300-4400 AA) with ATN1. Interacts with RERE. Interacts (via EVH1 domains) with ENAH. Interacts (via cytoplasmic domain) with CTNNB1.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Nucleus Cytoplasm, perinuclear region.

Tissue Specificity:
Expressed in many epithelial and some endothelial and smooth muscle cells.

Post-translational modifications:
Undergoes proteolytic cleavage. The extracellular domain is cleaved off and the cytoplasmic domain (about 400 AA) shuttles to the nucleus.

SWISS:
Q14517

Gene ID:
2195

Database links:


Entrez Gene: 2195 Human

Entrez Gene: 14107 Mouse

Omim: 600976 Human

SwissProt: Q14517 Human

Unigene: 481371 Human

Unigene: 27365 Mouse



產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FAT) Polyclonal Antibody, Unconjugated (bs-4278R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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