| 產(chǎn)品編號 | bs-5729R |
| 英文名稱 | Rabbit Anti-LGALS3BP antibody |
| 中文名稱 | 可溶性半乳糖凝集素3結(jié)合蛋白抗體 |
| 別 名 | Mac 2 binding protein; MAC2BP; Tumor associated antigen 90K; 90K; 90K MAC 2 BP; Basement membrane autoantigen p105; Galectin 3 binding protein; L3 antigen; Lectin galactoside binding soluble 3 binding protein; M2BP; Mac 2 BP; Serum protein 90K; LG3BP_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學 細胞周期蛋白 腫瘤細胞生物標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 63kDa |
| 細胞定位 | 細胞外基質(zhì) 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LGALS3BP: 351-450/585 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
LGALS3BP is a ligand of galectin 3, and has been suggested to influence tumor proliferation and metastasis formation. Galectins and their ligands have been implicated in cell transformation and cancer metastasis, and found to have prognostic value. LGALS3BP also known as Mac 2 BP,and as 90K, is a highly glycosylated, secreted protein extensively studied in human cancer, which binds galectin 1, galectin 3 and galectin 7. High expression levels of this molecule are associated with a shorter survival, the occurrence of metastasis or a reduced response to chemotherapy in patients with different types of malignancy. The mechanisms underlying the prognostic significance of 90K and galectins in cancer are far from being understood, although they may be related to the ability of these proteins to interact and, to some extent, modulate cell cell and cell matrix adhesion and apoptosis. Function: Promotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells. Subunit: Homodimers and homomultimers. Subcellular Location: Secreted. Secreted, extracellular space, extracellular matrix. Tissue Specificity: Ubiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts. Similarity: Contains 1 BACK (BTB/Kelch associated) domain. Contains 1 BTB (POZ) domain. Contains 1 SRCR domain. SWISS: Q08380 Gene ID: 3959 Database links: Entrez Gene: 3959 Human Omim: 600626 Human SwissProt: Q08380 Human Unigene: 514535 Human |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with LGALS3BP polyclonal antibody, unconjugated (bs-5729R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-LGALS3BP Polyclonal Antibody, Unconjugated(bs-5729R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
