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Rabbit Anti-Nectin 3  antibody (bs-6181R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號 bs-6181R
英文名稱 Rabbit Anti-Nectin 3  antibody
中文名稱 連接蛋白3抗體
別    名 CD113; CDw113; DKFZP566B0846; FLJ90624; Nectin-3; Nectin3; Poliovirus receptor related 3; Poliovirus receptor related protein 3; Poliovirus receptor-related protein 3; PPR3; PRR3; PVRL 3; PVRL3; PVRL3_HUMAN; PVRR3.   
Specific References  (1)     |     bs-6181R has been referenced in 1 publications.
[IF=3.77] Sheldon R. Gordon. et al. Evidence that Nectin-3 is the soybean agglutinin binding protein on rat corneal endothelium cell surfaces. EXP EYE RES. 2022 Oct;223:109212  ICC ;  Rat.  
研究領域 免疫學  微生物學  細菌及病毒  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,Mouse (predicted: Rat,Rabbit,Cow)
產(chǎn)品應用 WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 55kDa
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Nectin 3: 431-549/549 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Nectins are immunoglobulin-like adhesion molecules that interact with afadin. Afadin is an actin filament-binding protein that connects nectins to the actin cytoskeleton. The nectin-afadin system organizes adherens junctions cooperatively with the cadherin-catenin system in epithelial cells. The nectin/PRR-family consists of four members, nectin-1, -2, -3 and -4. All the members of the nectin family have two or three slice variants. Nectin 3 is mainly expressed in testis and placental tissues and interacts in vivo with both long and short isoforms of afadin.

Function:
Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.

Subunit:
Cis- and trans-homodimer. Can form trans-heterodimers with PVRL1/nectin-1, PVRL2/nectin-2, PVR, IGSF4B/Necl-1 and with IGSF4. Interacts with MLLT4/afadin. Binds with low affinity to TIGIT.

Subcellular Location:
Cell membrane; Single-pass membrane protein (Potential).

Tissue Specificity:
Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.

Similarity:
Belongs to the nectin family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.

SWISS:
Q9NQS3

Gene ID:
25945

Database links:

Entrez Gene: 25945 Human

Entrez Gene: 58998 Mouse

Omim: 607147 Human

SwissProt: Q9NQS3 Human

SwissProt: Q9JLB9 Mouse

Unigene: 293917 Human

Unigene: 328072 Mouse



產(chǎn)品圖片
Sample: Epididymis (Mouse) Lysate at 40 ug Primary: Anti- Nectin 3 (bs-6181R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD
Sample: HUVEC(Human) Cell Lysate at 30 ug Primary: Anti- Nectin 3 (bs-6181R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD
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