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Rabbit Anti-NCKAP1  antibody (bs-6204R)  
~~~促銷(xiāo)代碼KT202411~~~
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產(chǎn)品編號(hào) bs-6204R
英文名稱 Rabbit Anti-NCKAP1  antibody
中文名稱 膜相關(guān)的蛋白質(zhì)HEM2抗體
別    名 NCKP1_HUMAN; Nck-associated protein 1; NAP 1; Membrane-associated protein HEM-2; p125Nap1; HEM2; KIAA0587; NAP1; NAP125;   
研究領(lǐng)域 腫瘤  細(xì)胞生物  神經(jīng)生物學(xué)  信號(hào)轉(zhuǎn)導(dǎo)  干細(xì)胞  細(xì)胞凋亡  轉(zhuǎn)錄調(diào)節(jié)因子  細(xì)胞膜蛋白  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
交叉反應(yīng) Human,Mouse (predicted: Rat,Pig,Cow,Chicken,Dog,GuineaPig,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 129kDa
細(xì)胞定位 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human NCKAP1: 1021-1128/1128 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Contributes to small GTPase binding activity. Involved in Rac protein signal transduction; positive regulation of Arp2/3 complex-mediated actin nucleation; and positive regulation of lamellipodium assembly. Located in extracellular exosome and focal adhesion. Part of SCAR complex. [provided by Alliance of Genome Resources, Apr 2022]

Function:
Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes.

Subcellular Location:
Cell membrane. Cell projection

Tissue Specificity:
Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle. Expressed in cells of various brain regions including Purkinje cells and dentate nucleus of the cerebellum, CA4 region and dentate gyrus of the hippocampus, and in frontal gray and white matter

Similarity:
Belongs to the HEM-1/HEM-2 family.

SWISS:
Q9Y2A7

Gene ID:
10787

Database links:

Entrez Gene: 10787 Human

Entrez Gene: 50884 Mouse

Entrez Gene: 58823 Rat

SwissProt: Q9Y2A7 Human

SwissProt: P28660 Mouse

SwissProt: P55161 Rat



產(chǎn)品圖片
Sample: Lane 1: Human HepG2 cell lysates Lane 2: Human HeLa cell lysates Lane 3: Human 293T cell lysates Primary: Anti-NCKAP1 (bs-6204R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 129 kDa Observed band size: 135 kDa
Sample: Skeletal muscle (Mouse) Lysate at 40 ug Primary: Anti- NCKAP1 (bs-6204R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 129 kD Observed band size: 129 kD
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