| 產(chǎn)品編號 | bs-6624R |
| 英文名稱 | Rabbit Anti-IHH antibody |
| 中文名稱 | HHG2抗體 |
| 別 名 | HHG2; Indian Hedgehog; CENPB_HUMAN; Major centromere autoantigen B; Centromere protein B; CENP-B. |
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Specific References (2) | bs-6624R has been referenced in 2 publications.
[IF=3.098] Xianda Cheet al. MicroRNA?1 regulates the development of osteoarthritis in a Col2a1?Cre?ERT2/GFPfl/fl?RFP?miR?1?Mousemodel of osteoarthritis through the downregulation of Indian hedgehog expression. Int J Mol Med
. 2020 Jul;46(1):360-370. IHC ; mouse.
[IF=2.728] Xijuan Liu et al. Chondrocyte suppression is mediated by miR‐129‐5p via GDF11/SMAD3 signaling in developmental dysplasia of the hip. J Orthop Res. 2020 Dec;38(12):2559-2572. WB ; Rabbit.
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| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 干細(xì)胞 轉(zhuǎn)錄調(diào)節(jié)因子 細(xì)胞表面分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse,Rabbit,Pig,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 45kDa |
| 細(xì)胞定位 | 細(xì)胞膜 細(xì)胞外基質(zhì) 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IHH: 161-260/411 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Ihh is an intercellular signalling peptide essential for a variety of patterning events during development. It binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Ihh induces the expression of parathyroid hormone-related protein (PTHRP) and is implicated in endochondral ossification, possibly regulating the balance between growth and ossification of the developing bones. Function: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP) (By similarity). Subcellular Location: Indian hedgehog protein N-product: Cell membrane; Lipid-anchor; Extracellular side (By similarity). Note=The N-terminal peptide remains associated with the cell surface (By similarity). Indian hedgehog protein C-product: Secreted, extracellular space (By similarity). Note=The C-terminal peptide diffuses from the cell (By similarity). Tissue Specificity: Expressed in embryonic lung, and in adult kidney and liver. Post-translational modifications: The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity (By similarity). Cholesterylation is required for N-product targeting to lipid rafts and multimerization (By similarity). Palmitoylated. N-palmitoylation is required for N-product multimerization and full activity. DISEASE: Defects in IHH are the cause of brachydactyly type A1 (BDA1) [MIM:112500]. BDA1 is an autosomal dominant disorder characterized by middle phalanges of all the digits rudimentary or fused with the terminal phalanges. The proximal phalanges of the thumbs and big toes are short. Defects in IHH are a cause of acrocapitofemoral dysplasia (ACFD) [MIM:607778]. ACFD is a disorder characterized by short stature of variable severity with postnatal onset. The most constant radiographic abnormalities are observed in the tubular bones of the hands and in the proximal part of the femur. Cone-shaped epiphyses or a similar epiphyseal configuration with premature epimetaphyseal fusion result in shortening of the skeletal components involved. Cone-shaped epiphyses were also present to a variable extent at the shoulders, knees, and ankles. Similarity: Belongs to the hedgehog family. SWISS: P07199 Gene ID: 3549 Database links: Entrez Gene: 3549 Human Entrez Gene: 16147 Mouse Omim: 600726 Human SwissProt: Q14623 Human SwissProt: P97812 Mouse Unigene: 654504 Human Unigene: 439736 Mouse Unigene: 8711 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IHH) Polyclonal Antibody, Unconjugated (bs-6624R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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