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Rabbit Anti-DNase I  antibody (bs-7651R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號(hào) bs-7651R
英文名稱 Rabbit Anti-DNase I  antibody
中文名稱 脫氧核糖核酸酶1抗體
別    名 RNASE1; Dornase alfa; Deoxyribonuclease 1; Deoxyribonuclease I; Deoxyribonuclease1; DeoxyribonucleaseI; DNASE 1; DNase I lysosomal; DNASE1; DNaseI; DNL 1; DNL1; DRNI; Human urine deoxyribonuclease I; DNAS1_HUMAN.  
Specific References  (1)     |     bs-7651R has been referenced in 1 publications.
[IF=8.786] Julia Elrod. et al. Murine scald models characterize the role of neutrophils and neutrophil extracellular traps in severe burns. FRONT IMMUNOL. 2023; 14: 1113948  IF ;  Mouse.  
研究領(lǐng)域 細(xì)胞生物  細(xì)胞凋亡  細(xì)胞周期蛋白  表觀遺傳學(xué)  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Rat (predicted: Mouse,Rabbit,Pig,Chicken,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 29kDa
細(xì)胞定位 細(xì)胞核 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human DNase I: 101-200/282 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Deoxyribonuclease I gene is approximately 3.2 kb long with 9 exons separated by 8 introns. In the form of a bovine pancreatic enzyme preparation, it occupies an important place in the history of protein chemistry and enzymology: it was the first enzyme to be recognized as specific for DNA; it was the first DNase to be crystallized; and it was the first DNase for which a specific protein inhibitor was characterized. DNase I is a Ca2+ and Mg2+ dependant endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo- and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis. DNase I is an endonuclease that hydrolyzes double-stranded or single stranded DNA preferentially at sites adjacent to pyrimidine nucleotides. The product of hydrolysis is a complex mixture of 5'-phosphate mononucleotides and oligonucleotides. In the presence of Mg ion, DNase I attacks each strand of DNA independently and the cleavage sites are random.

Subcellular Location:
Secreted. Nucleus envelope.

Tissue Specificity:
Principally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva.

Similarity:
Belongs to the DNase I family.

SWISS:
P24855

Gene ID:
1773

Database links:

Entrez Gene: 1773 Human

Omim: 125505 Human

SwissProt: P24855 Human

Unigene: 629638 Human



產(chǎn)品圖片
Sample: MOLT-4(Human) Cell Lysate at 30 ug Primary: Anti-DNase I (bs-7651R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD
Sample: MKN45(Human) Cell Lysate at 30 ug Primary: Anti-DNase I (bs-7651R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD
Paraformaldehyde-fixed, paraffin embedded (rat intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (DNase I ) Polyclonal Antibody, Unconjugated (bs-7651R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DNase I) Polyclonal Antibody, Unconjugated (bs-7651R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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