| 產(chǎn)品編號 | bs-8212R |
| 英文名稱 | Rabbit Anti-TEF4 antibody |
| 中文名稱 | 轉(zhuǎn)錄增強因子TEF4抗體 |
| 別 名 | Embryonic TEA domain containing factor; Etdf; ETEF 1; ETF; TEA domain family member 2; TEAD 2; TEAD-2; TEAD2; Tead2 protein (TEA domain family member 2 (Mapped), isoform CRA_b); TEAD2_HUMAN; TEF 4; TEF4; Transcriptional enhancer factor 4; Transcriptional enhancer factor TEF 4; Transcriptional enhancer factor TEF-4. |
| 研究領(lǐng)域 | 細胞生物 免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Rabbit,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 49kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TEA domain family member 2: 235-340/447 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
TEF-4 is strongly coexpressed with TEF-1 in mouse mitotic neuroblasts and is also detected in the gut and the nephrogenic region of the kidney. TEF-4 associates with the powerful transcriptional coactivator YAP65 to mediate mitogenic signals. In addition, TEF-4 promotes the activation of the CTP:phosphocholine cytidylyltransferase (CCT) alpha protein, which is the rate-limiting enzyme of phosphatidylcholine biosynthesis, by enhancing the transcriptional activity of Ets-1. Function: Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif. Subunit: Interacts with YAP1 and WWTR1/TAZ. Subcellular Location: Nucleus. Similarity: Contains 1 TEA DNA-binding domain. SWISS: Q15562 Gene ID: 8463 Database links: Entrez Gene: 8463 Human Entrez Gene: 21677 Mouse Omim: 601729 Human SwissProt: Q15562 Human SwissProt: P48301 Mouse Unigene: 515534 Human Unigene: 3019 Mouse Unigene: 130442 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Primary: Anti-TEF4 (bs-8212R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
Paraformaldehyde-fixed, paraffin embedded (mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TEF4) Polyclonal Antibody, Unconjugated (bs-8212R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TEF4) Polyclonal Antibody, Unconjugated (bs-8212R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
