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Rabbit Anti-MSX1  antibody (bs-8512R)  
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產(chǎn)品編號 bs-8512R
英文名稱 Rabbit Anti-MSX1  antibody
中文名稱 MSH同源蛋白1樣蛋白抗體
別    名 Msh homeobox 1 like protein; Homeobox 7; Homeobox protein Hox-7; Homeobox protein MSX 1; Homeobox protein MSX-1; Homeobox protein MSX1; Homeobox, msh like 1; Homeobox, msh-like 1; HOX 7; Hox 7.1; Hox-7; HOX7; Hox7.1; HYD 1; HYD1; msh (Drosophila) homeo box homolog 1 (formerly homeo box 7); Msh; msh homeo box 1; msh homeo box homolog 1; Msh homeobox 1; Msh homeobox 1-like protein; msh homeobox homolog 1 (Drosophila); msh homeobox homolog 1; MSH, Drosophila, Homolog of, 1; MSX 1; MSX1; MSX1_HUMAN; Muscle segment homeobox; Muscle segment homeobox, Drosophila, Homolog of, 1; OFC5; STHAG1.  
Specific References  (2)     |     bs-8512R has been referenced in 2 publications.
[IF=3.337] Linlin Yang. et al. Identification and Validation of MSX1 as a Key Candidate for Progestin Resistance in Endometrial Cancer. Oncotargets Ther. 2020; 13: 11669–11688  WB ;  Human.  
[IF=1.85] Mu, Hailong, et al. "The function of Msx1 gene in promoting meiosis of dairy goat male germline stem cells (mGSCs)." Cell Biochemistry and Function (2013).  Goat.  
研究領(lǐng)域 細(xì)胞生物  免疫學(xué)  神經(jīng)生物學(xué)  信號轉(zhuǎn)導(dǎo)  干細(xì)胞  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse (predicted: Rat,Cow,Dog)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=5μg /Test,IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 31kDa
細(xì)胞定位 細(xì)胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human MSX1: 151-297/297 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Acts as a transcriptional repressor. May play a role in limb-pattern formation. Acts in cranofacial development and specifically in odontogenesis. Expression in the developing nail bed mesenchyme is important for nail plate thickness and integrity.

Function:
Acts as a transcriptional repressor. May play a role in limb-pattern formation. Acts in cranofacial development and specifically in odontogenesis. Expression in the developing nail bed mesenchyme is important for nail plate thickness and integrity.

Subcellular Location:
Nucleus

Tissue Specificity:
Expressed in the developing nail bed mesenchyme.

Post-translational modifications:
Sumoylated by PIAS1, desumoylated by SENP1 (By similarity).

DISEASE:
Defects in MSX1 are the cause of tooth agenesis selective type 1 (STHAG1) [MIM:106600]. A form of selective tooth agenesis, a common anomaly characterized by the congenital absence of one or more teeth. Selective tooth agenesis without associated systemic disorders has sometimes been divided into 2 types: oligodontia, defined as agenesis of 6 or more permanent teeth, and hypodontia, defined as agenesis of less than 6 teeth. The number in both cases does not include absence of third molars (wisdom teeth). Tooth agenesis selective type 1 can be associated with orofacial cleft in some patients.
Note=MSX1 is deleted in some patients with Wolf-Hirschhorn syndrome (WHS). WHS results from sub-telomeric deletions in the short arm of chromosome 4.
Defects in MSX1 are the cause of Witkop syndrome (WITS) [MIM:189500]. WITS is a form of ectodermal dyslasia also called tooth-and-nail syndrome or dysplasia of nails with hypodontia. Ectodermal dysplasias (EDs) constitute a heterogeneous group of developmental disorders affecting tissues of ectodermal origin. EDs are characterized by abnormal development of two or more ectodermal structures such as hair, teeth, nails and sweat glands, with or without any additional clinical sign. Each combination of clinical features represents a different type of ectodermal dysplasia. Witkop syndrome is characterized by abnormalities largely limited largely to teeth (some of which are missing) and nails (which are poorly formed early in life, especially toenails). This condition is distinguished from anhidrotic ectodermal dysplasia by autosomal dominant inheritance and little involvement of hair and sweat glands. The teeth are not as severely affected.
Defects in MSX1 are the cause of non-syndromic orofacial cleft type 5 (OFC5) [MIM:608874]; also called non-syndromic cleft lip with or without cleft palate 5. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum.

Similarity:
Belongs to the Msh homeobox family.
Contains 1 homeobox DNA-binding domain.

SWISS:
P28360

Gene ID:
4487

Database links:

Entrez Gene: 4487 Human

Entrez Gene: 17701 Mouse

Entrez Gene: 81710 Rat

Entrez Gene: 692067 Rhesus monkey

Omim: 142983 Human

SwissProt: Q2VL88 Chimpanzee

SwissProt: O02786 Cow

SwissProt: P28360 Human

SwissProt: P13297 Mouse

SwissProt: Q2VL87 Rhesus monkey

Unigene: 424414 Human

Unigene: 256509 Mouse

Unigene: 18117 Rat



產(chǎn)品圖片
Sample: Utreus (Mouse) Lysate at 40 ug Hela Cell (Human) Lysate at 40 ug Siha Cell (Human) Lysate at 40 ug A673 Cell (Human) Lysate at 40 ug Primary: Anti- MSX1 (bs-8512R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 31 kD
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37鈩?? for 20 min; Incubation: Anti-MSX1 Polyclonal Antibody, Unconjugated(bs-8512R) 1:200, overnight at 4擄C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Positive control: Hela cells Concebtration: 5μg/10^6 cells Incubation conditions: Avoid light , 30 minutes on the ice.
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