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Rabbit Anti-BRN3B  antibody (bs-6985R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號 bs-6985R
英文名稱 Rabbit Anti-BRN3B  antibody
中文名稱 腦特定蛋白Brn3B抗體
別    名 BRN3B; class 4; transcription factor 2; Brain specific homeobox/POU domain protein 3B; Brain-3B; Brain-specific homeobox/POU domain protein 3B; Brn-3B; BRN3.2; Brn3b POU domain transcription factor; PO4F2_HUMAN; POU class 4 homeobox 2; POU domain; POU domain class 4 transcription factor 2; POU domain protein; POU4F2.  
研究領(lǐng)域 細胞生物  神經(jīng)生物學(xué)  信號轉(zhuǎn)導(dǎo)  干細胞  表觀遺傳學(xué)  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Mouse,Rat (predicted: Human,Pig,Sheep,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg /Test,IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 41kDa
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human BRN3B: 301-400/409 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The Brn family of transcription factors are found in a highly restricted subset of neurons and are critical to the early embryonic development of the central nervous system. Brn-1 and Brn-2 are class III POU (Pit-Oct-Unc) domain proteins, whereas Brn-3 is a class IV POU domain protein. Three Brn-3 proteins have been described and are designated Brn-3a, Brn-3b and Brn-3c. While Brn-3a and Brn-3c stimulate transcription, Brn-3b generally functions as a transcriptional repressor. However, Brn-3b, but not Brn-3a, has been shown to regulate the expression of the acetylcholine receptor. Interestingly, Brn-3a has two functional transactivating domains, one at the amino-terminus and one at the carboxy-terminus. Brn-2 is thought to be involved in smooth muscle cell development and differentiation.

Function:
Transcription factor. May play a role in determining or maintaining the identities of a small subset of visual system neurons.

Subcellular Location:
Nucleus speckle.

Tissue Specificity:
Brain. Seems to be specific to the retina.

Similarity:
Belongs to the POU transcription factor family. Class-4 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.

SWISS:
Q12837

Gene ID:
5458

Database links:
UniProtKB/Swiss-Prot: Q12837.2

產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BRN3B) Polyclonal Antibody, Unconjugated (bs-6985R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control: RSC96(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody:Rabbit Anti-BRN3B antibody(bs-6985R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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