| 產(chǎn)品編號 | bs-9429R |
| 英文名稱 | Rabbit Anti-TXNDC9 antibody |
| 中文名稱 | 胚胎干細(xì)胞相關(guān)蛋白TXNDC9抗體 |
| 別 名 | Thioredoxin domain containing 9; APACD; ATP binding protein associated with cell differentiation; ATP-binding protein associated with cell differentiation; ES cell related protein; PHLP3; Phosducin like protein 3; Protein 1 4; Protein 1-4; Thioredoxin domain containing 9; Thioredoxin domain containing protein 9; Thioredoxin domain-containing protein 9; TXND9_HUMAN; TXNDC9; TXNDC9 protein. |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 干細(xì)胞 細(xì)胞分化 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Chicken,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 26kDa |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TXNDC9: 151-226/226 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Thioredoxins comprise a family of small proteins that, by catalyzing the oxidation of disulfide bonds, participate in redox reactions throughout the cell. Proteins that contain thioredoxin domains do not necessarily convey the oxidative properties of thioredoxins, but generally function as disulfide isomerases that enzymatically rearrange disulfide bonds found in various proteins. TXNDC9 (thioredoxin domain-containing protein 9), also known as APACD (ATP-binding protein associated with cell differentiation), is a 226 amino acid protein that contains one thioredoxin domain and may be involved in cell differentiation events. The gene encoding TXNDC9 maps to human chromosome 2, which houses over 1,400 genes and comprises nearly 8% of the human genome. Harlequin icthyosis, a rare and morbid skin deformity, is associated with mutations in the ABCA12 gene, while the lipid metabolic disorder sitosterolemia is associated with defects in the ABCG5 and ABCG8 genes. Additionally, an extremely rare recessive genetic disorder, Alstr鰉 syndrome, is caused by mutations in the ALMS1 gene, which maps to chromosome 2. Subunit: Forms ternary complexes with the chaperonin TCP1 complex, spanning the cylindrical chaperonin cavity and contacting at least 2 subunits. Similarity: Contains 1 thioredoxin domain. SWISS: O14530 Gene ID: 10190 Database links: Entrez Gene: 10190 Human Entrez Gene: 98258 Mouse Omim: 612564 Human SwissProt: O14530 Human SwissProt: Q9CQ79 Mouse Unigene: 536122 Human Unigene: 28438 Mouse |
| 產(chǎn)品圖片 |
Sample: HepG2 Cell (Human) Lysate at 40 ug
Primary: Anti-TXNDC9 (bs-9429R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 26 kD
Observed band size: 28 kD
Tissue/cell: mouse fetal liver; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TXNDC9 Polyclonal Antibody, Unconjugated(bs-9429R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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