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Rabbit Anti-phospho-Calmodulin 1/2/3 (Ser102)  antibody (bs-8519R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號 bs-8519R
英文名稱 Rabbit Anti-phospho-Calmodulin 1/2/3 (Ser102)  antibody
中文名稱 磷酸化鈣調(diào)節(jié)素抗體
別    名 p-CaM I (Ser 102); Calmodulin 1/2/3(phospho S102); Calmodulin 1/2/3; CALM 1; CALM 2; CALM 3; CALM; CALM1; CALM2; CALM3; CALML2; Calmodulin 1; Calmodulin1; CAM 1; CAM 2; CAM 3; CaM; CAM I; CAM1; CAM2; CAM3; CAMB; CAMC; CAMI; CAMIII; DD132; PHKD; Phosphorylase kinase delta; Phosphorylase kinase delta subunit; Calmodulin1; Calmodulin 1; Calmodulin-1; Calmodulin2; Calmodulin 2; Calmodulin-2; Calmodulin3; Calmodulin 3; Calmodulin-3; CALM1_HUMAN.  
產(chǎn)品類型 磷酸化抗體 
研究領(lǐng)域 腫瘤  細胞生物  免疫學(xué)  信號轉(zhuǎn)導(dǎo)  轉(zhuǎn)錄調(diào)節(jié)因子  細胞表面分子  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Mouse (predicted: Human,Rat,Pig,Cow,Dog)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 16kDa
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthesised phosphopeptide derived from human CaM I around the phosphorylation site of Ser102: YI(p-S)AA 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Calmodulin consists of two glycoproteins, 34 and 39 kDa, sometimes designated epithelial antigen, epithelial specific antigen, and epithelial glycoprotein. The glycoproteins are located on the cell membrane surface and in the cytoplasm of virtually all epithelial cells with the exception of most squamous epithelia, hepatocytes, renal proximal tubular cells, gastric parietal cells and myoepithelial cells. Epithelial Calmodulin is found in the large majority of adenocarcinomas of most sites (50-100% in various studies; as well as neuroendocrine tumours, including small cell carcinoma. Renal cell carcinoma and hepatocellular carcinoma stain in about 30% of the cases. Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin Ca(2+) complex are a number of protein kinases and phosphatases. Calmodulin has four functional calcium binding sites.

Subunit:
Interacts with MYO1C and RRAD. Interacts with MYO10 (By similarity). Interacts with CEP97, CEP110, TTN/titin and SRY. Interacts with USP6; the interaction is calcium dependent. Interacts with CDK5RAP2. Interacts with SCN5A. Interacts with RYR1 and RYR2.

Subcellular Location:
Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Note=Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.

Post-translational modifications:
Ubiquitination results in a strongly decreased activity.
Phosphorylation results in a decreased activity.

Similarity:
Belongs to the calmodulin family. Contains 4 EF-hand domains.

SWISS:
P0DP23

Gene ID:
801

Database links:

Entrez Gene: 801 Human

Entrez Gene: 12313 Mouse

Entrez Gene: 24242 Rat

SwissProt: P0DP23 Human

SwissProt: P0DP26 Mouse

SwissProt: P0DP29 Rat




鈣離子調(diào)節(jié)素(calmodulin,CaM): 鈣調(diào)蛋白(CaM),是由一條多肽鏈組成的單體蛋白。可結(jié)合4個Ca2+,胞漿Ca2+濃度≥10-2mmol/L時,Ca2+與CaM結(jié)合,構(gòu)象改變而激活Ca2+ -CaM激酶。
產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-CaM I (Ser 102)) Polyclonal Antibody, Unconjugated (bs-8519R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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