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Rabbit Anti-HCF2  antibody (bs-9843R)  
~~~促銷(xiāo)代碼KT202411~~~
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產(chǎn)品編號(hào) bs-9843R
英文名稱(chēng) Rabbit Anti-HCF2  antibody
中文名稱(chēng) 肝素輔助因子II抗體
別    名 HC II; HCF2; HLS2; Leuserpin 2; LS2; Protease inhibitor leuserpin 2; SERPIND1; HEP2_HUMAN.  
研究領(lǐng)域 心血管  細(xì)胞生物  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
交叉反應(yīng) Mouse,Rat (predicted: Human)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 55kDa
細(xì)胞定位 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human HCF2/Heparin Cofactor II: 101-200/499 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The herpes simplex virus (HSV) infection is initiated by VP16, a viral transcription factor that activates the viral immediate-early (IE) genes.The anticoagulant action of heparin is dependent on plasma components termed heparin cofactors. The first of these to be well characterized was antithrombin III. Heparin Cofactor II is antigenically distinct from AT III. Heparin Cofactor II is normal in patients with AT III deficiency and is low in patients with disseminated intravascular coagulation. It is expressed predominantly in liver. Its function is: thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, Heparin Cofactor II becomes the predominant thrombin inhibitor in place of antithrombin III (AT III). Also inhibits chymotrypsin, but in a glycosaminoglycan independent manner and peptides at the N terminal of Heparin Cofactor II have chemotactic activity for both monocytes and neutrophils. The N terminal acidic repeat region mediates, in part, the glycosaminoglycan accelerated thrombin inhibition. Defects in SERPIND1 are the cause of Heparin Cofactor II deficiency. Heparin Cofactor II deficiency is an important risk factor for hereditary thrombophilia, a multifactorial trait characterized by recurrent thrombosis and abnormal platelet aggregation in response to various agents. Heparin Cofactor II deficiency is inherited as an autosomal dominant disorder, in which affected individuals are prone to develop serious spontaneous thrombosis.

Function:
Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.
Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils.

Subcellular Location:
Secreted

Tissue Specificity:
Expressed predominantly in liver. Also present in plasma.

Post-translational modifications:
Phosphorylation sites are present in the extracellular medium.

DISEASE:
Defects in SERPIND1 are the cause of thrombophilia due to heparin cofactor 2 deficiency (THPH10) [MIM:612356]. A hemostatic disorder characterized by a tendency to recurrent thrombosis.

Similarity:
Belongs to the serpin family.

SWISS:
Q9Y5Z7

Gene ID:
29915

Database links:

Entrez Gene: 29915 Human

Omim: 607926 Human

SwissProt: Q9Y5Z7 Human

Unigene: 506558 Human



產(chǎn)品圖片
Sample: Lane 1: Mouse Testis tissue lysates Lane 2: Rat Testis tissue lysates Primary: Anti-HCF2 (bs-9843R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kDa Observed band size: 85 kDa
Paraformaldehyde-fixed, paraffin embedded (rat intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HCF2) Polyclonal Antibody, Unconjugated (bs-9843R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HCF2) Polyclonal Antibody, Unconjugated (bs-9843R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HCF2) Polyclonal Antibody, Unconjugated (bs-9843R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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