| 產(chǎn)品編號 | bs-9456R |
| 英文名稱 | Rabbit Anti-SIRT5 antibody |
| 中文名稱 | 沉默調(diào)節(jié)蛋白5抗體 |
| 別 名 | NAD dependent deacetylase sirtuin 5; NAD-dependent deacetylase sirtuin 5; NAD-dependent deacetylase sirtuin-5; Silent mating type information regulation 2 S.cerevisiae homolog 5; Sir2 like 5; Sir2-like 5; SIR2-like protein 5; SIR2L5; Sirt5; SIRT5_HUMAN; Sirtuin type 5. |
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Specific References (1) | bs-9456R has been referenced in 1 publications.
[IF=1.11] Takumida, Masaya, Hiroshi Takumida, and Matti Anniko. "Localization of sirtuins in the mouse inner ear." Acta Oto-Laryngologica 0 (2014): 1-8. IHC-F ; Mouse.
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| 研究領(lǐng)域 | 腫瘤 心血管 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 30kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SIRT5: 101-200/310 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Sirtuins (SIRT1-7) are human homologs of the yeast Sir2 (silent information regulator-2) protein and are divided into four main classes: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. In S. cerevisiae, Sir2 deacetylates histones in an NAD-dependent manner, which regulates silencing at the telomeric, rDNA (ribosomal RNA) and silent mating-type loci. The human SIRT proteins are NAD-dependent deacetylases that act as intracellular regulators and are thought to have ribosyltransferase activity. SIRT5 (NAD-dependent deacetylase sirtuin-5), also known as SIR2L5, is a 310 amino acid member of the class III sirtuins. Localized to mitochrondria and expressed throughout the body, SIRT5 is an NAD-dependent deacetylase that may link metabolic aging processes in humans. SIRT5 contains one deacetylase-sirtuin-type domain and can be deactivated by suramin, a drug that blocks the binding of various growth factors. Two isoforms of SIRT5 exist due to alternative splicing events. Subcellular Location: Mitochondrion matrix. Mitochondrion intermembrane space. Tissue Specificity: Widely expressed. SWISS: Q9NXA8 Gene ID: 23408 Database links: Entrez Gene: 23408 Human Entrez Gene: 68346 Mouse Omim: 604483 Human SwissProt: Q9NXA8 Human SwissProt: Q8K2C6 Mouse SwissProt: Q5R6G3 Orangutan Unigene: 567431 Human Unigene: 594133 Human Unigene: 35325 Mouse |
| 產(chǎn)品圖片 |
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-SIRT5 (bs-9456R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 33 kD
Blank control: mouse splenocytes(blue)
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
