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Rabbit Anti-ILVBL  antibody (bs-11014R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號 bs-11014R
英文名稱 Rabbit Anti-ILVBL  antibody
中文名稱 乙酰乳酸合成酶蛋白抗體
別    名 Acetolactate synthase homolog; Acetolactate synthase like protein; Acetolactate synthase-like protein; AHAS; ILV2H; 209L8; IlvB (bacterial acetolactate synthase) like; IlvB like protein; IlvB-like protein; Ilvbl; ILVBL_HUMAN.  
研究領(lǐng)域 心血管  細胞生物  免疫學  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human (predicted: Mouse,Rat,Pig,Dog,Horse)
產(chǎn)品應用 WB=1:500-2000,Flow-Cyt=3ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 68kDa
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ILVBL: 301-400/632 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 ILVBL is a 632 amino acid single-pass membrane protein that belongs to the TPP enzyme family. Expressed in the majority of tissues, ILVBL has the highest level of expression in heart, pancreas and placenta. ILVBL is highly homologous to several bacterial enzymes, including the B isozyme of the large catalytic subunit of E. coli acetohydroxy-acid synthase (AHAS) and the oxalyl-coA decarboxylase of O. formigenes, that utilize thiamine pyrophosphate as a cofactor. ILVBL binds one magnesium ion and one thiamine pyrophosphate per subunit, and may catalyze the initial step in branched-chain amino acid biosynthesis. The gene encoding ILVBL maps to human chromosome 19p13.12 and mouse chromosome 10 C1.

Subcellular Location:
Membrane.

Tissue Specificity:
Expressed in all tissues tested, with highest expression in heart, pancreas and placenta.

Similarity:
Belongs to the TPP enzyme family.

SWISS:
A1L0T0

Gene ID:
10994

Database links:

Entrez Gene: 10994 Human

Omim: 605770 Human

SwissProt: A1L0T0 Human

Unigene: 78880 Human



產(chǎn)品圖片
Sample: HepG2 Cell (Human) Lysate at 30 ug Primary: Anti-ILVBL (bs-11014R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68kD Observed band size: 68kD
Sample: Hcclm3(Human) Cell Lysate at 30 ug MCF-7(Human) Cell Lysate at 30 ug A431(Human) Cell Lysate at 30 ug Primary: Anti- ILVBL (bs-11014R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 68 kD
Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-ILVBL antibody (bs-11014R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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