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Rabbit Anti-Ceramide glucosyltransferase  antibody (bs-8593R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號(hào) bs-8593R
英文名稱 Rabbit Anti-Ceramide glucosyltransferase  antibody
中文名稱 葡萄糖神經(jīng)酰胺合成酶抗體
別    名 CEGT_HUMAN; UGCG; GCS; GLCT 1; GLCT-1; GLCT1; Glucosylceramide synthase; UDP glucose ceramide glucosyltransferase; UDP glucose N acylsphingosine D glucosyltransferase; UDP-glucose ceramide glucosyltransferase; UDP-glucose:N-acylsphingosine D-glucosyltransferase; Ugcg.  
Specific References  (2)     |     bs-8593R has been referenced in 2 publications.
[IF=5.923] Pietro Barbacini. et al. Novel Insight into the Serum Sphingolipid Fingerprint Characterizing Longevity. Int J Mol Sci. 2022 Jan;23(5):2428  WB ;  Human.  
[IF=4.757] Tami Igarashi. et al. Horse-Derived Ceramide Accentuates Glucosylceramide Synthase and Ceramide Synthase 3 by Activating PPARβ/δ and/or PPARγ to Stimulate Ceramide Synthesis. BIOMEDICINES. 2023 Feb;11(2):548  WB ;  Human.  
研究領(lǐng)域 腫瘤  細(xì)胞生物  細(xì)胞凋亡  細(xì)胞分化  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 45kDa
細(xì)胞定位 細(xì)胞漿 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human UGCG: 31-98/394 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Glucosylceramide synthase (GCS), also designated ceramide glucosyltransferase, belongs to the glycosyltransferase 2 family. It is a widely expressed integral membrane protein encoded by UGCG. The enzyme can be found in the plasma membrane of all eukaryotic cells, and a significant concentration of glucosylceramide synthase activity has been reported in the Golgi complex. Glucosylceramide synthase catalyzes the first glycosylation step in glycosphingolipid biosynthesis and functions as a glucosyltransferase and flippase in the transfer of glucose to ceramide. Glucosylceramide synthase operates in cell recognition, cell proliferation and differentiation, immune recognition and signal transduction. The regulation of ceramide levels through glucosylceramide synthase has been associated with the induction of apoptosis and notable research implicates this relationship with drug-induced apoptosis in a variety of cell types.

Function:
Catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. May also serve as a "flippase".

Subcellular Location:
Golgi apparatus membrane.

Tissue Specificity:
Found in all tissues examined.

Similarity:
Belongs to the glycosyltransferase 2 family.

SWISS:
Q16739

Gene ID:
7357

Database links:

Entrez Gene: 7357 Human

Entrez Gene: 22234 Mouse

Entrez Gene: 83626 Rat

Omim: 602874 Human

SwissProt: Q16739 Human

SwissProt: O88693 Mouse

SwissProt: Q9R0E0 Rat

Unigene: 304249 Human

Unigene: 593014 Human

Unigene: 198803 Mouse

Unigene: 24091 Rat



產(chǎn)品圖片
Sample: Lymph node (Mouse) Lysate at 40 ug Primary: Anti-Ceramide glucosyltransferase (bs-8593R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 45 kD
Sample: Small intestine (Mouse) Lysate at 40 ug Primary: Anti- Ceramide glucosyltransferase (bs-8593R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 45 kD
Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ceramide glucosyltransferase) Polyclonal Antibody, Unconjugated (bs-8593R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ceramide glucosyltransferase) Polyclonal Antibody, Unconjugated (bs-8593R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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