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Rabbit Anti-AAMP  antibody (bs-11388R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號 bs-11388R
英文名稱 Rabbit Anti-AAMP  antibody
中文名稱 血管內(nèi)皮細胞遷移蛋白抗體
別    名 angio-associated migratory cell protein; AAMP_HUMAN.  
研究領域 腫瘤  心血管  細胞生物  細胞粘附分子  血管內(nèi)皮細胞  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Dog)
產(chǎn)品應用 IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 47kDa
細胞定位 細胞漿 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human AAMP: 76-125/434 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 AAMP is a 434 amino acid immunoglobulin-like protein that contains 8 WD repeats. Expressed in endothelial cells, cytotrophoblasts and blood vessels, AAMP is thought to have a heparin-sensitive role in cell adhesion and cell migration. AAMP is strongly expressed in poorly differentiated colon adenocarcinoma cells, suggesting a role for AAMP in tumor progression.

Function:
The gene product of AAMP is an immunoglobulin-type protein, which is found to be expressed strongly in endothelial cells, cytotrophoblasts, and poorly differentiated colon adenocarcinoma cells found in lymphatics. The protein contains a heparin-binding domain and mediates heparin-sensitive cell adhesion.

Subcellular Location:
Cell Membrane and Cytoplasmic

Tissue Specificity:
Expressed in metastatic melanoma, liver, skin, kidney, heart, lung, lymph node, skeletal muscle and brain, and also in A2058 melanoma cells and activated T-cells (at protein level). Expressed in blood vessels. Strongly expressed in endothelial cells, cytotrophoblasts, and poorly differentiated. colon adenocarcinoma cells found in lymphatics.

Similarity:
Contains 8 WD repeats.

SWISS:
Q13685

Gene ID:
14

Database links:

Entrez Gene: 14 Human

Entrez Gene: 227290 Mouse

Entrez Gene: 301512 Rat

Omim: 603488 Human

SwissProt: Q3SZK1 Cow

SwissProt: Q13685 Human



產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AAMP Polyclonal Antibody, Unconjugated (bs-11388R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Blank control(blue): Raji(fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-AAMP antibody(bs-11388R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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