| 產(chǎn)品編號 | bs-11839R |
| 英文名稱 | Rabbit Anti-DNTNP antibody |
| 中文名稱 | 背神經(jīng)管核蛋白抗體 |
| 別 名 | DNTNP; Dorsal neural-tube nuclear protein; FA53A_HUMAN; FAM53A; family with sequence similarity 53, member A; Protein FAM53A. |
| 研究領域 | 細胞生物 發(fā)育生物學 神經(jīng)生物學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Dog (predicted: Chicken) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 43kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DNTNP/FAM53A: 255-340/398 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Dorsal neural-tube nuclear protein is a 398 amino acid nuclear protein. DNTNP is thought to play an important role in neural development by specifying dorsal cell fates within the neural tube. Widely expressed in the dorsal neural tube, DNTNP is most highly expressed in the dorsal regions of the midbrain, the hindbrain, the diencephalon, and the spinal neural tube and is expressed at lower levels in the branchial arches, the telencephalon, the heart, and somites of developing embryos. DNTNP is encoded by a gene located on chromosome 4 which contains many genes including the Huntingtin gene, which is found to encode an expanded glutamine tract in cases of Huntington's disease. Function: May play an important role in neural development; the dorsomedial roof of the third ventricle. Subcellular Location: Nucleus. Subnuclear distribution. Similarity: Belongs to the FAM53 family. SWISS: Q6NSI3 Gene ID: 152877 Database links: Entrez Gene: 152877 Human SwissProt: Q6NSI3 Human Unigene: 143314 Human |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DNTNP) Polyclonal Antibody, Unconjugated (bs-11839R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (dog testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DNTNP) Polyclonal Antibody, Unconjugated (bs-11839R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
