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Rabbit Anti-NFIB  antibody (bs-11899R)  
~~~促銷代碼KT202411~~~
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說明書: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包裝/詢價

產(chǎn)品編號 bs-11899R
英文名稱 Rabbit Anti-NFIB  antibody
中文名稱 核因子1B抗體
別    名 CCAAT Box Binding Transcription Factor; CCAAT-box-binding transcription factor; CTF; NF-I/B; NF1-B; NF1B; NF1B2; NFI-B; NFI-RED; NFIB; NFIB_HUMAN; NFIB3; Nuclear factor 1 B-type; Nuclear factor 1/B; Nuclear Factor 1B; Nuclear Factor I B; nuclear factor I/B; TGGCA Binding Protein; TGGCA-binding protein; NFIB2; NFIB3; HMGIC/NFIB.  
Specific References  (1)     |     bs-11899R has been referenced in 1 publications.
[IF=4.36] Hanyue Ying. et al. CircATL2 enhances paclitaxel resistance of ovarian cancer via impacting miR-506-3p/NFIB axis. 2021 Sep 19  WB ;  human.  
研究領(lǐng)域 細(xì)胞生物  神經(jīng)生物學(xué)  表觀遺傳學(xué)  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow,Chicken)
產(chǎn)品應(yīng)用 WB=1:500-2000,Flow-Cyt=1ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 47kDa
細(xì)胞定位 細(xì)胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human NFIB/NF1B2: 1-100/420 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Function:
Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Subunit:
Binds DNA as a homodimer.

Subcellular Location:
Nucleus.

Similarity:
Belongs to the CTF/NF-I family.
Contains 1 CTF/NF-I DNA-binding domain.

SWISS:
O00712

Gene ID:
4781

Database links:

Entrez Gene: 4781 Human

Entrez Gene: 18028 Mouse

Entrez Gene: 29227 Rat

Entrez Gene: 396209 Chicken

Omim: 600728 Human

SwissProt: P17924 Chicken

SwissProt: P13622 Hamster

SwissProt: O00712 Human

SwissProt: P97863 Mouse

Unigene: 644095 Human

Unigene: 317947 Mouse



產(chǎn)品圖片
Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-NFIB (bs-11899R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 48 kD
Sample:293T Cell (Human) Lysate at 40 ug Primary: Anti-NFIB (bs-11899R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 47 kD
Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug Lane 2: A431 (Human) Cell Lysate at 30 ug Lane 3: Lung (Mouse) Lysate at 40 ug Lane 4: Ovary (Mouse) Lysate at 40 ug Lane 5: Kidney (Mouse) Lysate at 40 ug Primary: Anti-NFIB (bs-11899R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 47 kD
Blank control: A431. Primary Antibody (green line): Rabbit Anti-NFIB antibody (bs-11899R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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