| 產(chǎn)品編號 | bs-4985R |
| 英文名稱 | Rabbit Anti-IGF1R antibody |
| 中文名稱 | 胰島素樣生長因子1受體抗體 |
| 別 名 | Insulin-like growth factor 1 receptor; CD221; CD221 antigen; IGF1 Receptor; IGF 1 receptor; IGF 1R; IGF I receptor; IGFIRC; Insulin like growth factor 1 receptor precursor; JTK13; MGC142170; MGC142172; Insulin-like growth factor 1 receptor beta chain; IGF 1 receptor; IGF-1R; IGF I receptor; IGF-I receptor; IGF-IR; IGF1R_HUMAN. |
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Specific References (6) | bs-4985R has been referenced in 6 publications.
[IF=6.208] Yanan Hao. et al. Alginate Oligosaccharides Repair Liver Injury by Improving Anti-Inflammatory Capacity in a Busulfan-Induced Mouse Model. INT J MOL SCI. 2023 Jan;24(4):3097
[IF=5.195] Chao Ma. et al. Anti-cervical cancer effects of Compound Yangshe granule through the PI3K/AKT pathway based on network pharmacology. J ETHNOPHARMACOL. 2022 Oct;:115820 WB ; Mouse.
[IF=3.86] Chu, Meiqiang, et al. "MicroRNA-126 participates in lipid metabolism in mammary epithelial cells." Molecular and Cellular Endocrinology (2017). WB ; Human.
[IF=3.082] Xiaolan Ouyang. et al. Transplantation of Human Amnion Epithelial Cells Improves Endometrial Regeneration in Rat Model of Intrauterine Adhesions. Stem Cells Dev. 2020 Oct;29(20):1346-1362 IHC ; Human.
[IF=3.038] Pengfei Fu. et al. Baicalein Ameliorates Epilepsy Symptoms in a Pilocarpine-Induced Rat Model by Regulation of IGF1R. Neurochem Res. 2020 Dec;45(12):3021-3033 WB ; Rat.
[IF=1.84] Zhao, Yong, et al. "Inhibition of peripubertal sheep mammary gland development by cysteamine through reducing progesterone and growth factor production." Theriogenology (2016). WB ; Sheep.
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| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 生長因子和激素 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 糖尿病 細(xì)胞膜蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Pig,Sheep,Cow) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IGF1R/CD221 beta chain: 821-920/1367 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. [provided by RefSeq, Jul 2008]. Function: This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin. Subunit: Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. Forms a hybrid receptor with INSR, the hybrid is a tetramer consisting of 1 alpha chain and 1 beta chain of INSR and 1 alpha chain and 1 beta chain of IGF1R. Interacts with ARRB1 and ARRB2. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibrobasts, spleen and placenta (at protein level). Expressed in a variety of tissues. Post-translational modifications: The cytoplasmic domain of the beta subunit is autophosphorylated on tyrosine residues in response to insulin-like growth factor I (IGF I). Phosphorylation of Tyr-980 is required for IRS1- and SHC1-binding. DISEASE: Defects in IGF1R may be a cause in some cases of resistance to insulin-like growth factor 1 (IGF1 resistance) [MIM:270450]. IGF1 resistance is a gowth deficiency disorder characterized by intrauterine growth retardation and poor postnatal growth accompanied with increased plasma IGF1. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. Contains 3 fibronectin type-III domains. Contains 1 protein kinase domain. SWISS: P08069 Gene ID: 3480 Database links: Entrez Gene: 3480 Human Entrez Gene: 16001 Mouse Omim: 147370 Human SwissProt: P08069 Human SwissProt: Q60751 Mouse Unigene: 643120 Human Unigene: 714012 Human Unigene: 275742 Mouse Unigene: 10957 Rat |
| 產(chǎn)品圖片 |
Sample:Kidney (Mouse) Lysate at 40 ug
Primary: Anti- IGF1R (bs-4985R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69/150 kD
Observed band size: 69 kD
Blank control(blue): Mouse Spleen(fixed with 2% paraformaldehyde for 10 min at 37℃). Primary Antibody:Rabbit Anti-IGF1R antibody (bs-4985R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Positive control: (mo)Splenocytes(2% Paraformaldehyde-fixed )
Isotype Control Antibody: Rabbit IgG;
Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA
Secondary Antibody: Goat anti-rabbit IgG-FITC;
Dilution: 1:200 in 1 X PBS containing 0.5% BSA
Primary Antibody: rabbit Anti-IGF1R bs-4985R;
Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA
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